Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jason Gardiner
Date created: 2008-10-28 12:00:00
Date modified: 2015-05-08 01:09:44
lacIq->BisdB
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K123009_sequence (Version 1) |
Description
This Construct will allow for the detection and degredation of BPA in cells already containing the ER (K123003) biobrick. It works in two systems first. Together K123003 and K123009 produce a NOT gate where when Bisphenol A (BPA), Estrogen or any other estrogen mimic is in the system the product of K123003 dimerizes and attaches to the ERE site of K123009. This stops the production of TetR (R0040) by phsically stoping the Polymerase from transcribing it. This allows the pTet(C0040)promoter to turn on the production of RFP (E1010) for detection as well as BisdA(K123000) and BisdB(K123001)which allow for the degredation of BPA.Notes
While BisdA and BisdB have only been shown to degrade BPA we have put in RFP to allow for the detection of other estrogen mimics as well.Source
Bisphenolics| Access | Instance | Definition |
| public public public public | BBa_K123002 BBa_R0040 BBa_E1010 BBa_K123008 | BBa_K123002 p(tetR) mRFP1 BBa_K123008 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K123002 BBa_R0040 BBa_E1010 BBa_K123008 | 1,742 751,804 811,1516 1525,3266 | BBa_K123002 p(tetR) mRFP1 BBa_K123008 |