Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shoujie Sun
Date created: 2014-10-03 11:00:00
Date modified: 2015-05-08 01:10:03
Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1361002_sequence (Version 1) |
Description
This part contains the major subunit of curli fiber CsgA and its nucleator CsgBtrunc, in which CsgA is constitutive expression whereas CsgBtrunc is under the control of T7 promotor.This part cannot functioning alone without expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.
Notes
The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG.Source
CsgAC and CsgBtrunc were collected by PCR from genome of E coli DH5alpha respectively.| Access | Instance | Definition |
| public public public public public public | BBa_K206000 BBa_B0034 BBa_K1361995 BBa_B0015 BBa_K608003 BBa_K1361998 | pBAD BBa_B0034 BBa_K1361995 BBa_B0015 BBa_K608003 BBa_K1361998 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K206000 BBa_B0034 BBa_K1361995 BBa_B0015 BBa_K608003 BBa_K1361998 | 1,130 139,150 157,555 564,692 701,756 763,1609 | pBAD BBa_B0034 BBa_K1361995 BBa_B0015 BBa_K608003 BBa_K1361998 |