BBa_K145217

BBa_K145217 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K145217
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonas Demeulemeester
Date created: 2008-08-19 11:00:00
Date modified: 2015-05-08 01:10:29

FILTER Complete



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K145217_sequence (Version 1)

Description

FILTER This device was designed to filter out noise in an input signal; in our case OmpF PoPs. If there is a short increase in transcription from this promoter, a small amount of J23066 RiboKey is produced. This is then used to unlock (J23078) the short lived UmuD-tagged T7 RNA polymerase. This results in an increase of active T7 protein.

The final part of this filter is an AND gate which is used in the components further on in the system. This AND gate consists of a T7 RNA polymerase promoter with another RiboLock (J23078 again). The AND gate will only function if the duration of the noise is sufficiently long and intense (= a signal) in order to have both the short-lived RiboKey AND T7 polymerase available at the same time and thus to produce the component.

Notes

We did a lot of modeling on this device.
The most efficient Berkeley 2006 key and lock have been used.
The RiboKy might be required in higher copy number although modeling doesn't predict this.

Source

Other parts + synthetic

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K145217/1