Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonas Demeulemeester
Date created: 2008-08-20 11:00:00
Date modified: 2015-07-09 01:34:23
CELL DEATH
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K145230_sequence (Version 1) |
Description
A hybrid promoter controls the production of LuxR and ccdB. The promoter is activated by HSL-LuxR complex and repressed by P22 c2. The LuxR coding region is preceded by a strong RBS and followed by a bad terminator (60% efficiency). This means there is read through into the ccdB coding region which can be translated from a very weak RBS.If the P22 c2 repressor is absent, there should be a strong enough LuxR background to allow autoactivation if HSL is added. The amound of ccdB should be sublethal as long as there is no significant activation.
Notes
The ratio of fully activated transcription to background transcription is the sole determinant of the ratio of ccdB produced in the activated state to the background state. Modeling has shown that this should be ok.Source
other parts| Access | Instance | Definition |
| public public public public public public public | BBa_K145150 BBa_B0034 BBa_C0062 BBa_B0014 BBa_B0033 BBa_K145151 BBa_B0015 | BBa_K145150 BBa_B0034 luxr BBa_B0014 BBa_B0033 ccdB BBa_B0015 |