Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Iowis Zhu
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:44
pBAD-RBS-OmpA-Bovine Galectin-1-B0012 Terminator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1489004_sequence (Version 1) |
Description
A fusion protein of OmpA (BBa_K1489002) fused to Bovine Galectin-1 (BBa_K1489001). Used to bind sugars at the outer cell membrane. Under pBAD regulation.Notes
When designing this part, had to choose between the pBAD and pLac promoters. Decided to use the pBAD promoter due to tighter regulation; we were unsure of whether the protein was toxic and did not want to have too much leaky expression.Source
OmpA is from E. coli genomeBovine galectin 1 is from Bos taurus genome.
| Access | Instance | Definition |
| public public public public public | BBa_K206000 BBa_B0034 BBa_K1489002 BBa_K1489001 BBa_B0012 | pBAD BBa_B0034 BBa_K1489002 BtGal1 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K206000 BBa_B0034 BBa_K1489002 BBa_K1489001 BBa_B0012 | 1,130 139,150 159,622 629,1045 1054,1094 | pBAD BBa_B0034 BBa_K1489002 BtGal1 BBa_B0012 |