BBa_K1532006

BBa_K1532006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1532006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xinyuan Qiu

Date created: 2014-09-29 11:00:00

Date modified: 2015-05-08 01:10:52

Plac->LuxR

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K1532006_sequence (Version 1)

Description

This part is a functional device with the input of LacI & IPTG and the out put of the protein LuxR. The expression of the output signal will be activated if the protein LacI and the small molecule IPTG exists the same time. This part may not work when the glucose exists in the culture.

Notes

The BBa_R0010 we used in the device contains a cup-site so that the glucose may affect the work of this device. So, glucose-free culture is suggested to be used if you want to use this device.

Source

This part is assembled with standard bio-bricks from the distribution with the standard assembly protocol.

Components

Access	Instance	Definition
public	BBa_K1532001	BBa_K1532001
public	BBa_R0010	LacI
public	BBa_B0030	BBa_B0030
public	BBa_C0062	luxr
public	BBa_B0015	BBa_B0015
public	BBa_K1532001	BBa_K1532001

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1532001	1,10	BBa_K1532001
BBa_R0010	19,218	LacI
BBa_B0030	227,241	BBa_B0030
BBa_C0062	248,1003	luxr
BBa_B0015	1037,1165	BBa_B0015
BBa_K1532001	1174,1183	BBa_K1532001

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1532006/1

Member of these Collections

• iGEM Parts Registry