Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Astrid Deryckere
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 11:29:08
RBS-LuxI-His-RBS-LuxR-E-Term-Plux
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1709006_sequence (Version 1) |
Description
Autoinducer synthetase for acyl-homoserine lactones (AHL): LuxI synthesizes AHL (3OC6HSL). Two molecules of AHL form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri. In absence of AHL, the right Lux promotor only gives weak constitutive expression of downstream genes. Any gene you would like to be controlled by AHL and thus cell density can be cloned after this complex.Notes
For the ease of cloning, the sequence was devided in multiple gBlocks.Therefor, there is a mistake in the composite part sequence since the scar is only partly present.
Source
The Lux operon originates from V. Fischeri.| Access | Instance | Definition |
| public public public public public | BBa_K1709001 BBa_J61100 BBa_K1709004 BBa_B1002 BBa_R0062 | BBa_K1709001 BBa_J61100 BBa_K1709004 BBa_B1002 lux pR |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1709001 BBa_J61100 BBa_K1709004 BBa_B1002 BBa_R0062 | 1,627 636,647 654,1454 1463,1496 1505,1559 | BBa_K1709001 BBa_J61100 BBa_K1709004 BBa_B1002 lux pR |