BBa_K182101

BBa_K182101 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K182101

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Zuzana Hruskova and Calum de Burgh

Date created: 2009-07-06 11:00:00

Date modified: 2015-05-08 01:11:06

pLux-Lac Hybrid with GFP Reporter and Double Terminator

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K182101_sequence (Version 1)

Description

Hybrid Promoter with two LacO sites and pLux-Lac sites for Quorum Sensing reporting via GFP.

Lux promoter is foremost followed by first operator, then pLac and second LacO site.

Comprised of parts BBa_I751502 upstream and BBa_E0840 downstream.

Notes

N/A atm

Source

Ligation of BBa_I751502 upstream and BBa_E0840 downstream.

Components

Access	Instance	Definition
public	BBa_I751502	BBa_I751502
public	BBa_B0030	BBa_B0030
public	BBa_E0040	GFP
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I751502	1,74	BBa_I751502
BBa_B0030	83,97	BBa_B0030
BBa_E0040	104,823	GFP
BBa_B0010	832,911	BBa_B0010
BBa_B0012	920,960	BBa_B0012

Other Properties

• igem#experience: Issues
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K182101/1

Member of these Collections

• iGEM Parts Registry