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BBa_K182101

BBa_K182101 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K182101

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Zuzana Hruskova and Calum de Burgh

Date created: 2009-07-06 11:00:00

Date modified: 2015-05-08 01:11:06

pLux-Lac Hybrid with GFP Reporter and Double Terminator

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Details

Types	DnaRegion
Roles	Reporter engineered_region
Sequences	BBa_K182101_sequence (Version 1)

Description

Hybrid Promoter with two LacO sites and pLux-Lac sites for Quorum Sensing reporting via GFP.

Lux promoter is foremost followed by first operator, then pLac and second LacO site.

Comprised of parts BBa_I751502 upstream and BBa_E0840 downstream.

Notes

N/A atm

Source

Ligation of BBa_I751502 upstream and BBa_E0840 downstream.

Components

Access	Instance	Definition
public public public public public	BBa_I751502 BBa_B0030 BBa_E0040 BBa_B0010 BBa_B0012	BBa_I751502 BBa_B0030 GFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I751502 BBa_B0030 BBa_E0040 BBa_B0010 BBa_B0012	1,74 83,97 104,823 832,911 920,960	BBa_I751502 BBa_B0030 GFP BBa_B0010 BBa_B0012

Other Properties

igem#experience	Issues
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K182101/1