BBa_K1886012

BBa_K1886012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1886012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lejian Jiang
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-18 03:41:48

AND GATE-input_DPD+green light



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K1886012_sequence (Version 1)

Description

The circuit to receive input signals is composed of AND GATE, quorum sensing promoter and light-controlled promoter. After being induced by DPD, plsrA promoter activates the expression of T7 RNA polymerase which contains two amber mutations (T7ptag) . After being induced by green light, PcpcG2 promoter activates the expression of a special tRNA (supD), which is used to identify amber mutation. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase. Therefore, when DPD and green light exist together, T7 RNA polymerase will be expressed, and then T7 promoter will be activated to express the downstream gene.

Notes

PT7(BBa_K1321338) contains T7 promoter and RBS(B0034, which enables easy addition to any coding part which does not already have these parts.

Source

This part is a combination of DPD input and Green light input. The subparts of this part is from iGEM Distribution Kits.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1886012/1