BBa_K191008

BBa_K191008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K191008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Le Thanh Tu NGUYEN

Date created: 2009-10-18 11:00:00

Date modified: 2015-05-08 01:11:15

Promoter LacI - RBS - LovTAP(ILE427PHE) - Term

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K191008_sequence (Version 1)

Description

Complete coding sequence for mutated protein LOVTAP (mutation site: a.a #427 ILE is changed into PHE), with inducible promoter, RBS site and terminator.

Notes

Mutagenesis kit of Stratagene was used.

Source

Site specific mutation on plasmid with part BBa_K191003

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_B0030	BBa_B0030
public	BBa_K191009	BBa_K191009
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_B0030	209,223	BBa_B0030
BBa_K191009	230,916	BBa_K191009
BBa_B0010	925,1004	BBa_B0010
BBa_B0012	1013,1053	BBa_B0012

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K191008/1

Member of these Collections

• //function/sensor/light
• iGEM Parts Registry