BBa_K2055016

BBa_K2055016 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2055016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Raul Garza & Carlos Vasquez

Date created: 2016-09-27 11:00:00

Date modified: 2016-09-28 05:20:43

Part designed for verification and characterization of the FUR/LacI inverter

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K2055016_sequence (Version 1)

Description

E. coli constitutive promoter with Ferric Uptake Regulator & LacI Inverter. Activates RFP expression in presence of iron

Notes

LacI and FUR proteins are optimized for Acidithiobacillus ferrooxidans. It has two RBS after the constitutive promoter and before the FUR CDS.

Source

Composite part

Components

Access	Instance	Definition
public	BBa_J23110	BBa_J23110
public	BBa_B0032	BBa_B0032
public	BBa_B0034	BBa_B0034
public	BBa_K2055009	BBa_K2055009
public	BBa_K2055010	BBa_K2055010
public	BBa_B0034	BBa_B0034
public	BBa_K2055007	BBa_K2055007
public	BBa_B0012	BBa_B0012
public	BBa_R0011	lacI+pL
public	BBa_B0034	BBa_B0034
public	BBa_E1010	mRFP1
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23110	1,35	BBa_J23110
BBa_B0032	44,56	BBa_B0032
BBa_B0034	65,76	BBa_B0034
BBa_K2055009	83,562	BBa_K2055009
BBa_K2055010	571,589	BBa_K2055010
BBa_B0034	598,609	BBa_B0034
BBa_K2055007	616,1701	BBa_K2055007
BBa_B0012	1710,1750	BBa_B0012
BBa_R0011	1759,1812	lacI+pL
BBa_B0034	1822,1833	BBa_B0034
BBa_E1010	1840,2545	mRFP1
BBa_B0010	2554,2633	BBa_B0010
BBa_B0012	2642,2682	BBa_B0012

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2055016/1

Member of these Collections

• iGEM Parts Registry