Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Muhammad Ismail
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 12:36:12
Carbon Monoxide sensor device (pCooF-Blue Chromoprotein)
| Types | DnaRegion |
| Roles | Project engineered_region |
| Sequences | BBa_K2056003_sequence (Version 1) |
Description
This part is the carbon monoxide (CO) sensing module of the 'Inspector NOxCO' device. 'Inspector NOxCO' is a bacterial sensor for carbon monoxide (CO) and oxides of nitrogen (NOx) in vehicle emissions.This part consists of the Anderson constitutive promoter(BBa_J23110) and RBS(B0030) with the coding sequence for CooA(BBa_K352001) a CO dependent transcriptional activator and single terminator(B0010) followed by CooA activated strong promoter pCooF(K352002) with RBS(B0030) and 'amilCP, blue chromoprotein' with terminator(BBa_B0012).
If CO is present, the constitutive expressed CooA will bind to it and then bind to the pCOoF promoter activating transcription of the blue chromoprotein.
Notes
The selection of the RBS and Constitutive promoter. Because of limitations of repeats that could be synthesized, we had to give up on the double terminators in the original design and go with single different terminators. A 70bp spacer(B0040) has been added for transcriptional efficiency.Source
All parts in this sequence come from the Registry and have been listed. However, the complete part has been synthesized from IDT using their free gBLocks offer.| Access | Instance | Definition |
| public public public public public public public public public | BBa_J23110 BBa_B0030 BBa_K352001 BBa_B0010 BBa_B0040 BBa_K352002 BBa_B0030 BBa_K592009 BBa_B0012 | BBa_J23110 BBa_B0030 BBa_K352001 BBa_B0010 spacer BBa_K352002 BBa_B0030 amilCP BBa_B0012 |