Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Guosheng Zhang
Date created: 2009-09-19 11:00:00
Date modified: 2015-05-08 01:11:33
Sal->rbs->T7ptag->Terminator
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K228227_sequence (Version 1) |
Description
This is a branch of the AND GATE built by Peking University 2009 iGEM Team. The addition of salicylate can induce the transcription of T7ptag. However, if the SupD tRNA is absent, T7ptag might not be translated into functional T7 polymerase normally because of some amber stop codon mutations in it. We also have constructed a few similar parts with various RBS, which would make the expression regulation of T7ptag gene more convenient by choosing different parts.Source
Built by joining different standardized biobricks together| Access | Instance | Definition |
| public public public public public | BBa_K228004 BBa_B0030 BBa_K228000 BBa_B0010 BBa_B0012 | BBa_K228004 BBa_B0030 BBa_K228000 BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K228004 BBa_B0030 BBa_K228000 BBa_B0010 BBa_B0012 | 1,1052 1061,1075 1082,3754 3763,3842 3851,3891 | BBa_K228004 BBa_B0030 BBa_K228000 BBa_B0010 BBa_B0012 |