BBa_K243021

BBa_K243021 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K243021

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Freiburg Bioware09

Date created: 2009-10-17 11:00:00

Date modified: 2015-05-08 01:11:37

DsbA-His-DigA-Split Linker-Fok_a

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K243021_sequence (Version 1)

Description

The DsbA tag is used to transfer the produced protein into the periplasm. This makes is easier for us to extract the protein.

Notes

none.

Source

Combined by serial cloning steps.

Components

Access	Instance	Definition
public	BBa_K243002	DsbA
public	BBa_B0105	Scar 25
public	BBa_K157011	His
public	BBa_B0105	Scar 25
public	BBa_K243003	DigA
public	BBa_B0105	Scar 25
public	BBa_K157009	linker
public	BBa_K243000	Fok_a

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K243002	1,54	DsbA
BBa_B0105	55,60	Scar 25
BBa_K157011	61,78	His
BBa_B0105	79,84	Scar 25
BBa_K243003	85,636	DigA
BBa_B0105	637,642	Scar 25
BBa_K157009	643,693	linker
BBa_K243000	694,1272	Fok_a

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K243021/1

Member of these Collections

• iGEM Parts Registry