Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Juan Sim??n ??lamos
Date created: 2012-09-21 11:00:00
Date modified: 2015-05-08 01:13:09
psb1C3_IntKR recombination plasmid with RFP under psbA2 promoter
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K743016_sequence (Version 1) |
Description
The psb1C3_IntKR recombination plasmid allows the neutral integration of any desired sequence into Synechocystis PCC6803 chromosome.Composite part BBa_K743016 is designed for the expression of RFP under the control of the Synechocystis psbA2 promoter.
Notes
This composite part is not intended to be used for standard biobrick assembly because of a EcoR1 restriction site in psb1C3_intK plasmid. You should use gibson assembly.Source
psbA2 was amplified from Synechocystis genomic DNA and its sequence was obtained from BBa_K390017.RFP is from blubliblo
For more information about the plasmid backbone, check psb1C3_IntKR recombination plasmid page.
| Access | Instance | Definition |
| public public public public public | BBa_K743000 BBa_K390017 BBa_E1010 BBa_S05073 BBa_K743001 | RS1 BBa_K390017 mRFP1 BBa_S05073 RS2 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K743000 BBa_K390017 BBa_E1010 BBa_S05073 BBa_K743001 | 1,522 523,609 610,1315 1316,2419 2420,3106 | RS1 BBa_K390017 mRFP1 BBa_S05073 RS2 |