Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrea Olson
Date created: 2011-04-17 11:00:00
Date modified: 2015-05-08 01:13:55
Complete Part for ROSE Regulated Kill Gene
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M1205_sequence (Version 1) |
Description
This gene is meant to be used as a regulatory gene to prevent accidental spread of any experiment cultures that may be produced, either in the lab or in DIY kits. It works by utilizing a temperature sensitive ROSE sequence that creates a hairpin structure that prevents translation of a tetracycline repressor. This allows for the suicide gene to be coded for and results in the death of the cell. When conditions are above 42C, the hairpin bonds are broken and the tetracycline repressor is now coded, repressing the kill switch and allowing for the continual growth of the culture.Notes
-Must take into consideration the half life of the repressor produced at above 42C. If the culture is then submitted to a lower temperature, how long does it take for the repressor proteins present to degrade and allow the kill gene to be translated.Source
-The ROSE sequence was taken from the RNA thermometer in Bradyrhizobium japonicum that initiates translation at 42??C.-Kill Switch General Design and Idea
http://2010.igem.org/Team:HKU-Hong_Kong
| Access | Instance | Definition |
| public public public public public public public public public public public public public public public public public | BBa_I742101 BBa_J22106 BBa_J61100 BBa_K115001 BBa_C0040 BBa_B0010 BBa_R0040 BBa_J61102 BBa_E0040 BBa_K112805 BBa_K112806 BBa_B0015 BBa_J23116 BBa_J61101 BBa_K112807 BBa_B0010 BBa_I742102 | difF BBa_J22106 BBa_J61100 BBa_K115001 tetR BBa_B0010 p(tetR) BBa_J61102 GFP BBa_K112805 BBa_K112806 BBa_B0015 BBa_J23116 BBa_J61101 BBa_K112807 BBa_B0010 difR |