Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

iGEM to SBOL conversion
igem2sbol Version 1 (Activity)
Conversion of the iGEM parts registry to SBOL2.1

SyBiOntKB to SBOL conversion
SyBiOntKB2sbol Version 1 (Activity)
Conversion of the Synthetic Biology Ontology knowledge base to SBOL 2.1

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

Composite Sequence Pull
Composite Version 1 (Activity)
When multiple sources where combined to make a new part.

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

BBa_K549023
BBa_K549023 Version 1 (Component)
10x His-tag fused to pabA

BBa_J04031
BBa_J04031 Version 1 (Component)
GFP w/LVA tag

GC Optimised Sequence Pull
GC_Optimised Version 1 (Activity)
Used to indicate that a sequence was GC optimised after pulling from the source and before submission

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

BBa_K137006
BBa_K137006 Version 1 (Component)
pabB (from pABA synthesis)

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

HA
BBa_I712008 Version 1 (Component)
HA affinity tag

NLS
BBa_J176008 Version 1 (Component)
NLS

BBa_K549022
BBa_K549022 Version 1 (Component)
pabA with fused His-tag

BBa_K105012
BBa_K105012 Version 1 (Component)
10 aa flexible protein domain linker

PEST
BBa_J176018 Version 1 (Component)
PEST

linker
BBa_K133132 Version 1 (Component)
8 aa protein domain linker

BBa_K549027
BBa_K549027 Version 1 (Component)
10x His-tag fused to petE2

OmpA
BBa_K103006 Version 1 (Component)
OmpA outer membrane protein A fused to linker; displays proteins on cell surface

NLSmycSTOP
BBa_J176031 Version 1 (Component)
NLS myc stop

BBa_K1150016
BBa_K1150016 Version 1 (Component)
HA tag

pabA
BBa_K137005 Version 1 (Component)
pabA (from pABA synthesis)

BBa_K1223006
BBa_K1223006 Version 1 (Component)
His-Tag

BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS_rev) -- corresponds to BBa_B0030

BBa_K549026
BBa_K549026 Version 1 (Component)
petE2 with the fused 10x His-tag

CD4 signal
BBa_I712009 Version 1 (Component)
CD4 signal peptide; localizes to plasma membrane

Hin
BBa_J31000 Version 1 (Component)
DNA-invertase Hin from Salmonella typhimurium

RFC105 A
BBa_K1362414 Version 1 (Component)
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A

BBa_K1162009
BBa_K1162009 Version 1 (Component)
10x-Histidine (10x-His) Tag with Met (ATG) For N-terminal purification

BBa_K549019
BBa_K549019 Version 1 (Component)
10x His-tag

BBa_K137110
BBa_K137110 Version 1 (Component)
pabA + pabB complete pABA construct

RE
BBa_J3101 Version 1 (Component)
Recombinational Enhancer (RE) for Hin/Hix inverting

G-His6(N)
BBa_K1362430 Version 1 (Component)
C-terminal His6 (N-Intein)

BBa_K726003
BBa_K726003 Version 1 (Component)
T7 promoter + lac operator + His tag

BBa_K416003
BBa_K416003 Version 1 (Component)
Yeast Secretion Tag