BBa_K395110
BBa_K395110 Version 1 (Component)
GFP reporter repressed by LasR and 3OC12HSL (K395025:K121013)

BBa_K2055015
BBa_K2055015 Version 1 (Component)
Promoter + Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of Fe

BBa_K2055019
BBa_K2055019 Version 1 (Component)
Part designed for verification and characterization of the heat the shock promoter and Inverter.

BBa_K176172
BBa_K176172 Version 1 (Component)
Repressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.15->luxR+pLux/Tet->GFP

BBa_K185033
BBa_K185033 Version 1 (Component)
An inverter of a special lactose operon system based on J23110-rbs34-lacI-dter-plac-rbs31

BBa_K880003
BBa_K880003 Version 1 (Component)
Asymmetrically digestible reporter, same orientation of inverted repeats as K137058 (external sites

BBa_K1994042
BBa_K1994042 Version 1 (Component)
sgRNA with dCas9 binding site Sequence 6 and PP7 inserted

BBa_K176115
BBa_K176115 Version 1 (Component)
Cell density controller: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)

BBa_K872000
BBa_K872000 Version 1 (Component)
Signal converter, converts a HCO3- signal in a cAMP intermediate signal

BBa_K872002
BBa_K872002 Version 1 (Component)
Signal converter, converts a HCO3- signal in a cAMP intermediate signal

BBa_K176091
BBa_K176091 Version 1 (Component)
Cell density controller: PoPS->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

BBa_K542006
BBa_K542006 Version 1 (Component)
pBAD Inverse-Regulated Arg-tagged ECFP and EYFP (FRET Reporter)

BBa_K1132037
BBa_K1132037 Version 1 (Component)
AND-inverted RFP gate (BBa_K1132034) with T7 polymerase under the control of a strong promoter

BBa_K1132038
BBa_K1132038 Version 1 (Component)
XOR-inverted RFP gate (BBa_K1132035) with T7 polymerase under the control of a strong promoter

BBa_K176249
BBa_K176249 Version 1 (Component)
Low input score automatic selector w/o lethal device(with reporter): PoPS->mRFP->CI-LVA+pCI->K176240

BBa_K1621009
BBa_K1621009 Version 1 (Component)
pOP - protein expression vector compatible with RFC[25]

BBa_K1045015
BBa_K1045015 Version 1 (Component)
RBS BBa_B0034 with inversed Pre- and Suffix- Promoter reverse - Promoter - DarR operator - BBa_E0240

BBa_K106697
BBa_K106697 Version 1 (Component)
AarI AD acceptor vector (pRS315, Cyc1P, Adh1t-8XLexA Ops)

BBa_K1124123
BBa_K1124123 Version 1 (Component)
inverse PCR template for creating new sRNA (plambda-micC sRNA scaffold-terminator)

BBa_K127003
BBa_K127003 Version 1 (Component)
Polystyrene binding peptide inserted CPX generated by qurum sensing and constitutive GFP genarator

BBa_K106679
BBa_K106679 Version 1 (Component)
GFP, under pheromone-inducible promoter, 5' LexA Ops

BBa_M36459
BBa_M36459 Version 1 (Component)
PoPS -> Cell Lysis

BBa_M36458
BBa_M36458 Version 1 (Component)
PoPS -> Cell Lysis

BBa_K542008
BBa_K542008 Version 1 (Component)
pLacI Regulated Lumazine Synthase and pBAD Inverse-Regulated Arg-tagged ECFP and EYFP

BBa_M34567
BBa_M34567 Version 1 (Component)
converts PoPS to foobob

BBa_K395102
BBa_K395102 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395005:K121013)

BBa_K395103
BBa_K395103 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395006:K121013)

BBa_K987001
BBa_K987001 Version 1 (Component)
This is a composite part which has the function to invert the temperature activation by the part: BB

BBa_K1452000
BBa_K1452000 Version 1 (Component)
Pos/neg selection (Bacillus)

BBa_R4030
BBa_R4030 Version 1 (Component)
PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.