mCherry
mCherry Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

iGEM Feature Libraries
igem_feature_libraries_collection Version 1 (Unknown)
A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Free Genes Feature Libraries
free_genes_feature_libraries_collection Version 1 (Unknown)
A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

BBa_B0014
BBa_B0014 Version 1 (Component)
double terminator (B0012-B0011)

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_I13507
BBa_I13507 Version 1 (Component)
Screening plasmid intermediate

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

BBa_J06602
BBa_J06602 Version 1 (Component)
Construction Intermediate: mCherry, bacterial with RBS (B0034.J06504)

BBa_I13504
BBa_I13504 Version 1 (Component)
Screening plasmid intermediate

mCherry
BBa_J06702 Version 1 (Component)
mCherry, bacterial with RBS and forward terminator

BBa_E0430
BBa_E0430 Version 1 (Component)
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)

BBa_K2066527
BBa_K2066527 Version 1 (Component)
B0034 with scar TACTAG

BBa_E0422
BBa_E0422 Version 1 (Component)
ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)

mCherry
BBa_J06505 Version 1 (Component)
monomeric RFP optimized for bacteria

pT7
BBa_K525998 Version 1 (Component)
Promoter T7 and RBS

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

BBa_E0420
BBa_E0420 Version 1 (Component)
ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)

BBa_J06603
BBa_J06603 Version 1 (Component)
Construction Intermediate: mCherry, bacterial (LVA+) with RBS (B0034.J06505)

lacI
BBa_Q04121 Version 1 (Component)
QPI (B0034.C0012.B0015.R0011)

T7 RBS
BBa_K1362090 Version 1 (Component)
strong T7 RBS

mCherry
BBa_E2060 Version 1 (Component)
derivative of mRFP1, yeast-optimized

BBa_I13500
BBa_I13500 Version 1 (Component)
Screening plasmid intermediate

tetR
BBa_Q04400 Version 1 (Component)
QPI (B0034.C0040.B0015.R0040)

BBa_B0035
BBa_B0035 Version 1 (Component)
RBS (B0030 derivative)

BBa_Q04510
BBa_Q04510 Version 1 (Component)
QPI (B0034.C0051.B0015.R0051)

BBa_K592016
BBa_K592016 Version 1 (Component)
B0034-YF1-B0034-FixJ, blue light sensor and RR with RBS

BBa_J06801
BBa_J06801 Version 1 (Component)
QPI (B0034.J06501.B0015.R0011)

EYFP
BBa_E0034 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_C0261
BBa_C0261 Version 1 (Component)
AHL-making Enzyme, luxI (+RBS)

BBa_J06550
BBa_J06550 Version 1 (Component)
Construction intermediate: lambda cI with RBS (B0034.C0051)

BBa_K773003
BBa_K773003 Version 1 (Component)
mCherry-LVA

RBS_cassette
RBS_cassette Version 1 (Component)
AvrII restriction site (cctagg), overlapped with RBS (aggagga)

BBa_E0432
BBa_E0432 Version 1 (Component)
EYFP (RBS+ LVA+ TERM) (B0034.E0032.B0015)

BBa_B0024
BBa_B0024 Version 1 (Component)
double terminator (B0012-B0011), reversed

BBa_J06572
BBa_J06572 Version 1 (Component)
Construction intermediate: cI857 (E07700) with RBS (B0034.J06502)

RBS-spoVG
BBa_K143021 Version 1 (Component)
SpoVG ribosome binding site (RBS) for B. subtilis