Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
BBa_B0032BBa_B0032 Version 1 (Component)RBS.3 (medium) -- derivative of BBa_0030
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
BBa_J61101BBa_J61101 Version 1 (Component)Ribosome Binding Site Family Member
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
BBa_J61120BBa_J61120 Version 1 (Component)Ribosome Binding Site Family Member
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_B0033BBa_B0033 Version 1 (Component)RBS.4 (weaker) -- derivative of BBa_0030
BBa_J15001BBa_J15001 Version 1 (Component)strong synthetic E. coli ribosome binding site with SacI site.
hixCBBa_J44000 Version 1 (Component)hixC binding site for Salmonella typhimurium Hin recombinase
BBa_J61100BBa_J61100 Version 1 (Component)Ribosome Binding Site Family Member
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
BBa_J85600BBa_J85600 Version 1 (Component)attB1 recombination site
Restriction_sitesRestriction_sites Version 1 (Component)SacI - KpnI - SmaI - BamHI - XbaI - SalI - PstI - SphI - HindIII - NotI
BBa_M31333BBa_M31333 Version 1 (Component)g3 to BamHI site
AdapterBBa_I732007 Version 1 (Component)BglII + BamHI
BBa_K1638016BBa_K1638016 Version 1 (Component)8 aa linker without BamHI restriction site and TEV recognition site
BBa_J44001BBa_J44001 Version 1 (Component)Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030
RBS_cassetteRBS_cassette Version 1 (Component)AvrII restriction site (cctagg), overlapped with RBS (aggagga)
loxBBa_J61046 Version 1 (Component)[Lox] site for recombination
CD4 signalBBa_I712009 Version 1 (Component)CD4 signal peptide; localizes to plasma membrane
BBa_I13457BBa_I13457 Version 1 (Component)Improved insertion site with two RNAse E cut sites
RBS-spoVGBBa_K143021 Version 1 (Component)SpoVG ribosome binding site (RBS) for B. subtilis
BBa_I13452BBa_I13452 Version 1 (Component)Adds another BioBrick site
BamHI rsBBa_K792013 Version 1 (Component)BamHI restriction site (standalone)
BBa_K165002BBa_K165002 Version 1 (Component)Kozak sequence (yeast RBS)
PBS_F24PBS_F24 Version 1 (Component) BBa_K909007BBa_K909007 Version 1 (Component)TetR DNA binding domain containing BamHI site for protein fusions
T7 RBSBBa_K1362090 Version 1 (Component)strong T7 RBS
BBa_I766217BBa_I766217 Version 1 (Component)Stop-D-BamHI-Adh Terminator Adaptor
BBa_J54033BBa_J54033 Version 1 (Component)PrefixII_SalI,BamHI
EcoRIEcoRI Version 1 (Component) NheINheI Version 1 (Component) GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission
PacIPacI Version 1 (Component) SpeISpeI Version 1 (Component) GFPBBa_K1638008 Version 1 (Component)linker-GFP without BamHI restriction site