BBa_K1797013
BBa_K1797013 Version 1 (Component)
Exo-an exonuclease in the Red homologous recombination system

S+P28+ori+
BBa_K320009 Version 1 (Component)
Constitutive expression of P28 in Asaia and Resistance to Tetracycline

S+imm+ori+
BBa_K320008 Version 1 (Component)
Constitutive expression of Immunotoxin in Asaia and resistance to Kanamycine

BBa_K1859002
BBa_K1859002 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GSGSGS linker

BBa_K1859005
BBa_K1859005 Version 1 (Component)
GSGSGS linker+ The 5??side of the intron [BBa_K1332003]

BBa_K364304
BBa_K364304 Version 1 (Component)
TRE-CMV

BBa_J58013
BBa_J58013 Version 1 (Component)
A periplasmic binding protein that docks a vanillin molecule

BBa_K1283000
BBa_K1283000 Version 1 (Component)
The protective melittin generator

BBa_K1828994
BBa_K1828994 Version 1 (Component)
A pH sensitive promoter

BBa_K2056003
BBa_K2056003 Version 1 (Component)
Carbon Monoxide sensor device (pCooF-Blue Chromoprotein)

BBa_M11203
BBa_M11203 Version 1 (Component)
HXT7 promoter for S. cerevisiae, repressed in the presence of glucose

Cys-Thr
BBa_K1787000 Version 1 (Component)
High Cys-Thr protein

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K733002
BBa_K733002 Version 1 (Component)
<i>xylR</i>+<i>PxylA</i>: A xylose inducible promoter with its transcriptional regulator.

BBa_K1051502
BBa_K1051502 Version 1 (Component)
Sic1 mutation, non stop codon

BBa_K1323999
BBa_K1323999 Version 1 (Component)
RBS of a part

SortA clv.
BBa_K1362454 Version 1 (Component)
Sortase A recognition/cleavage site

BBa_J69591
BBa_J69591 Version 1 (Component)
GFP reporter device - using Standard Promoter (1RPU)

BBa_K177031
BBa_K177031 Version 1 (Component)
mgtc testing device with phoQP from salmonella

BBa_K194001
BBa_K194001 Version 1 (Component)
GFP (a yeast- and FACS-optimized GFP variant),

BBa_K1333000
BBa_K1333000 Version 1 (Component)
M13 bacteriophage g1p with a FLAG tag on C-terminal

BBa_K1333003
BBa_K1333003 Version 1 (Component)
M13 bacteriophage g4p with a FLAG tag on C-terminal

BBa_K1333001
BBa_K1333001 Version 1 (Component)
M13 bacteriophage g2p with a FLAG tag on C-terminal

BBa_K2176001
BBa_K2176001 Version 1 (Component)
Cassette for the constitutive production of GAL4-KaiCp and LexA-SasA, linked by a self-cleaving P2A

SAMmt
BBa_K1312005 Version 1 (Component)
S-adenosyl methionine-dependent methyltransferase relate to Met-DMSP byosynthesis pathway(SAMmt)

BBa_J37034
BBa_J37034 Version 1 (Component)
LuxI added to GFP

BBa_J28000
BBa_J28000 Version 1 (Component)
Operon 1 (a)

BBa_K078001
BBa_K078001 Version 1 (Component)
A fragment that encodes an enzyme involed in dioxin degradation

BBa_K1719013
BBa_K1719013 Version 1 (Component)
a dasd

BBa_K200030
BBa_K200030 Version 1 (Component)
Lipase A from B.subtilis

RBS.cre
BBa_I716436 Version 1 (Component)
{rbs1-A}{GTG.Cre}

SP-aprE
BBa_K1674000 Version 1 (Component)
Secretion signal peptide originated from the B.subtilis extracellular protease AprE

BBa_M36567
BBa_M36567 Version 1 (Component)
Actuator to reduce Hg(II) to elemental Hg.

BBa_K137044
BBa_K137044 Version 1 (Component)
Device with promoter with (C)10 repeat

BBa_K727622
BBa_K727622 Version 1 (Component)
Rose Odor Generator Device (Geraniol)-Complete System

BBa_K204022
BBa_K204022 Version 1 (Component)
3O₆HSL generater device

BBa_K137045
BBa_K137045 Version 1 (Component)
Device with promoter with (C)12 repeat

BBa_J13203
BBa_J13203 Version 1 (Component)
3OC6HSL + LuxR reporter and aiia tuning device

BBa_J13204
BBa_J13204 Version 1 (Component)
3OC6HSL + LuxR reporter and cI tuning device

BBa_K2012002
BBa_K2012002 Version 1 (Component)
PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain.

SmtA
BBa_K1620007 Version 1 (Component)
Synechococcus metallothionenin A (SmtA)

BBa_K137115
BBa_K137115 Version 1 (Component)
Constitutively expresses cI, the lambda repressor.

BBa_K496001
BBa_K496001 Version 1 (Component)
trying to add

BBa_K2082237
BBa_K2082237 Version 1 (Component)
RFP and Tetracycline resistence(tetA) under the control of a modified lacZ promoter; Fusion protein

BBa_I715066
BBa_I715066 Version 1 (Component)
It's a testing part for RE's effect on GFP.

BBa_K611093
BBa_K611093 Version 1 (Component)
A Truncation of BBa_C0051

BBa_K422007
BBa_K422007 Version 1 (Component)
CheY (AarI A-part)

BBa_K1321100
BBa_K1321100 Version 1 (Component)
Phytochelatin (PC) EC20 fused to linker-dCBD with T7 promoter