BBa_K1179009
BBa_K1179009 Version 1 (Component)
(Cas9-VP16) A non-nuclease Cas9 with a C-terminal VP16 fusion for activation

BBa_K1407031
BBa_K1407031 Version 1 (Component)
a part of bistable system

BBa_K1407035
BBa_K1407035 Version 1 (Component)
a part of bistable system

BBa_K195621
BBa_K195621 Version 1 (Component)
pRE with repressor gene PenI(+LVA)

BBa_K1113411
BBa_K1113411 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome

BBa_K1510409
BBa_K1510409 Version 1 (Component)
A Steptococcus Mutants adhesive complex.

BBa_I12012
BBa_I12012 Version 1 (Component)
Reporter for the Barkai-Leibler oscillator

VanBP
BBa_J58105 Version 1 (Component)
Synthetic periplasmic binding protein that docks a vanillin molecule

BBa_M1838
BBa_M1838 Version 1 (Component)
RPS Tgene from Methilobacterium chloromeneticum

BBa_M1844
BBa_M1844 Version 1 (Component)
RPS Tgene from Methilobacteriun chloromeneticum

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BGH pA
BBa_K678019 Version 1 (Component)
BGH poly A, mammalian terminator

BBa_K313007
BBa_K313007 Version 1 (Component)
gfp generator in the reverse direction

oriTr/LacZ
BBa_K125350 Version 1 (Component)
oriTr with lac promoter and lacZ

BBa_K1497004
BBa_K1497004 Version 1 (Component)
B0034-CHS - Chalcone Synthase from Gerbera with strong RBS

BBa_K812057
BBa_K812057 Version 1 (Component)
A GoldenBricked version of sfGFP protein

BBa_K1148014
BBa_K1148014 Version 1 (Component)
promoter hlbA from L. bulgaricus

BBa_K313001
BBa_K313001 Version 1 (Component)
DNA invertase hin in the reverse direction

BBa_K1189015
BBa_K1189015 Version 1 (Component)
E coil with a GFP under a lacI promoters

BBa_K118012
BBa_K118012 Version 1 (Component)
Synthetic ribosome binding site added by Son of Babel procedure

BBa_K1789003
BBa_K1789003 Version 1 (Component)
GFP1(with termination codon)

BBa_K539623
BBa_K539623 Version 1 (Component)
a RBS (B0034)+ilvC

BBa_K336020
BBa_K336020 Version 1 (Component)
bacterial cellulose synthase B

BBa_K336021
BBa_K336021 Version 1 (Component)
bacterial cellulose synthase D

BBa_K336052
BBa_K336052 Version 1 (Component)
bacterial cellulose synthase C

nhaS
BBa_K1255000 Version 1 (Component)
nhaS gene that express NhaS, a putative sodium ion binding protein.

BBa_K539627
BBa_K539627 Version 1 (Component)
a RBS (B0032)+Alss

BBa_K883403
BBa_K883403 Version 1 (Component)
PLRV Coat Protein gene - Isolated from Desir??e potato

BBa_K1657002
BBa_K1657002 Version 1 (Component)
It is called TAB. It have the resistance to glyphosate and the 2, 4-Dichlorophenoxyacetic acid

BBa_K1657001
BBa_K1657001 Version 1 (Component)
It is called BAT. It have the resistance to glyphosate and the 2, 4-Dichlorophenoxyacetic acid

BBa_K1657004
BBa_K1657004 Version 1 (Component)
It is called TAB. It have the resistance to glyphosate and the 2, 4-Dichlorophenoxyacetic acid

BBa_K1657003
BBa_K1657003 Version 1 (Component)
It is called BAT. It have the resistance to glyphosate and the 2, 4-Dichlorophenoxyacetic acid

BBa_K091121
BBa_K091121 Version 1 (Component)
LacI wild-type gene

RBS.TetR
BBa_I15040 Version 1 (Component)
RBS.TetR from pASKIBA3

BBa_K1890030
BBa_K1890030 Version 1 (Component)
BolA gene with RBS and terminator

BBa_J24816
BBa_J24816 Version 1 (Component)
J23101 promoter with Luc gene attached

BBa_J24815
BBa_J24815 Version 1 (Component)
J23101 promoter with Renilla gene attached

BBa_K1547000
BBa_K1547000 Version 1 (Component)
PSAD Promoter and Terminator, with GFP reporter gene

BBa_M31517
BBa_M31517 Version 1 (Component)
Refactored M13KO7 genome from HpaI site in gene II through BamHI site in gene III

BBa_K1960000
BBa_K1960000 Version 1 (Component)
Sensing device with switch for gram-negative bacterium

BBa_K1159315
BBa_K1159315 Version 1 (Component)
TMD of SERK Receptor from <i>Physcomitrella patens</i> with C-terminal GFP fusion in RFC[25]

shTERT
BBa_K1722001 Version 1 (Component)
shTERT is a cancer cell specific promoter with high efficiency.

BBa_K1638017
BBa_K1638017 Version 1 (Component)
RBS from pKT25 and pUT18C with consensus Shine-Dalgarno

BBa_I711001
BBa_I711001 Version 1 (Component)
generates the tyrosinase for melanin biosynthesis

mnt (w)
BBa_C0073 Version 1 (Component)
mnt repressor (weak) from Salmonella phage P22 (+LVA)

BBa_K1686046
BBa_K1686046 Version 1 (Component)
Curdlan synthase gene with codon optimisation for E. coli

BBa_K2027046
BBa_K2027046 Version 1 (Component)
Bacterial Collagen with Homotrimeric Coiled-Coil, Elastin Cross-linker, and mRNA Circularization

BBa_K1336006
BBa_K1336006 Version 1 (Component)
LacI Expression Cassette with ispB RC gene

BBa_K109550
BBa_K109550 Version 1 (Component)
Bacterial conjugative regulator KorA

oriT/LacZ
BBa_K125360 Version 1 (Component)
oriT with lac promoter and lacZ