BBa_K102909
BBa_K102909 Version 1 (Component)
TA11 gate from synthetic algorithm v1.1

BBa_K102910
BBa_K102910 Version 1 (Component)
TA12 gate from synthetic algorithm v1.1

BBa_K157002
BBa_K157002 Version 1 (Component)
Transmembrane region of the EGF-Receptor (ErbB-1)

BBa_K836005
BBa_K836005 Version 1 (Component)
Lysozyme from Rhodococcus phage RER2 (codon usage optimized for R, opacus)

gpS
BBa_K836006 Version 1 (Component)
Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)

nitR
BBa_K836007 Version 1 (Component)
Nitrilase regulator from R. rhodochrous (codon usage optimized for R. opacus)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K098991
BBa_K098991 Version 1 (Component)
cI promoter with GFP reporter

BBa_S04138
BBa_S04138 Version 1 (Component)
cI QPI with GFP reporter system

BBa_K098988
BBa_K098988 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and high promoter

BBa_K098987
BBa_K098987 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and low promoter

BBa_K098982
BBa_K098982 Version 1 (Component)
lac inducible system with low promoter and GFP reporter

linker
BBa_K157013 Version 1 (Component)
15 aa flexible glycine-serine protein domain linker; Freiburg standard

BBa_K102911
BBa_K102911 Version 1 (Component)
TA13 gate from synthetic algorithm v1.2

rTT+pR
BBa_K093003 Version 1 (Component)
Convergent Promoter System: Forward Module: rTT+lambda pR

Q04510+RFP
BBa_K131018 Version 1 (Component)
Intermediate for the Response circuit

BBa_K101016
BBa_K101016 Version 1 (Component)
Dually repressed promoter with sites for TetR and P22MNT binding

BBa_J69522
BBa_J69522 Version 1 (Component)
tetR-repressible promoter coupled with mCherry Reporter

BBa_K165024
BBa_K165024 Version 1 (Component)
Sin3 repression domain + LexA DNA-binding domain

BBa_K165025
BBa_K165025 Version 1 (Component)
Kozak + Sin3 repression domain + LexA DNA-binding domain

BBa_K165026
BBa_K165026 Version 1 (Component)
Sin3 repression domain + Gli1 DNA-binding domain

BBa_K165027
BBa_K165027 Version 1 (Component)
Kozak + Sin3 repression domain + Gli1 DNA-binding domain

BBa_K156009
BBa_K156009 Version 1 (Component)
OFP (orange fluorescent protein)

SBFP2
BBa_K156010 Version 1 (Component)
SBFP2 (strongly enhanced blue fluorescent protein)

BBa_K102905
BBa_K102905 Version 1 (Component)
TA7 gate from synthetic algorithm v1.1

lexA
BBa_K165020 Version 1 (Component)
LexA Synthetic Activator (untagged)

BBa_K165021
BBa_K165021 Version 1 (Component)
Kozak + LexA Synthetic Activator (untagged)

BBa_K165022
BBa_K165022 Version 1 (Component)
Zif268-HIV synthetic activator (untagged)

BBa_K165023
BBa_K165023 Version 1 (Component)
Kozak + Zif268-HIV synthetic activator (untagged)

BBa_K125810
BBa_K125810 Version 1 (Component)
slr2016 signal sequence + GFP fusion for secretion of GFP

OriTR
BBa_J01003 Version 1 (Component)
OriT-R (Origin of transfer for the R-plasmid nic region)

pBSEP1
BBa_K090401 Version 1 (Component)
Gram-positive Shuttle Vector for Episomal Expression

BBa_K086001
BBa_K086001 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086003
BBa_K086003 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K165030
BBa_K165030 Version 1 (Component)
mCYC promoter plus Zif268-HIV binding sites

BBa_K165031
BBa_K165031 Version 1 (Component)
mCYC promoter plus LexA binding sites

BBa_I0466
BBa_I0466 Version 1 (Component)
RhlR Protein Generator

BBa_S04142
BBa_S04142 Version 1 (Component)
The Partial Butanol Operon (Parts I725021+I725022)

BBa_S04143
BBa_S04143 Version 1 (Component)
The Partial Butanol Operon (Parts I725023+I725024)

BBa_S04144
BBa_S04144 Version 1 (Component)
The Partial Butanol Operon (Parts I725024+I725025)

BBa_S04145
BBa_S04145 Version 1 (Component)
The Partial Butanol Operon (Parts I725021+I725022+I725023)

BBa_S04147
BBa_S04147 Version 1 (Component)
The Partial Butanol Operon (5 inserts from I725021 to I725025)

BBa_K156027
BBa_K156027 Version 1 (Component)
Genetic attenuator (synthetic transcription terminator) ~68% efficient

BBa_K165032
BBa_K165032 Version 1 (Component)
mCYC promoter plus Gli1 binding sites

BBa_K156028
BBa_K156028 Version 1 (Component)
Genetic attenuator (synthetic transciption terminator) ~45% efficient

BBa_K156029
BBa_K156029 Version 1 (Component)
Genetic attenuator (synthetic transcription terminator) ~17% efficient