BBa_K1696017
BBa_K1696017 Version 1 (Component)
tag3 for mf-lon to recognize

BBa_K1696019
BBa_K1696019 Version 1 (Component)
tag5 for mf-lon to recognize

BBa_K1696020
BBa_K1696020 Version 1 (Component)
tag6 for mf-lon to recognize

BBa_K1696018
BBa_K1696018 Version 1 (Component)
tag4 for mf-lon to recognize

BBa_K855003
BBa_K855003 Version 1 (Component)
coding sequence for pvdQ with histag added to the C-terminal followed by stop codon

BBa_K1323000
BBa_K1323000 Version 1 (Component)
sgRNA (1): CRISPR Silencing of YFP

BBa_K1323001
BBa_K1323001 Version 1 (Component)
sgRNA (2): CRISPR Silencing of YFP

BBa_K1189018
BBa_K1189018 Version 1 (Component)
Human ferritin di-subunit with E coil w/ LacI promoter

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K2057003
BBa_K2057003 Version 1 (Component)
LasR under the regulation of the promoter chiA74

BBa_M36146
BBa_M36146 Version 1 (Component)
Cold-inducible promoter for HeLa cells

BBa_K1381014
BBa_K1381014 Version 1 (Component)
Silencing sRNA (10 bases long) for USP45 (Based on Spot42)

BBa_K1381016
BBa_K1381016 Version 1 (Component)
Silencing sRNA (20 bases long) for USP45 (Based on Spot42)

BBa_K1381015
BBa_K1381015 Version 1 (Component)
Silencing sRNA (15 bases long) for USP45 (Based on Spot42)

BBa_K1407026
BBa_K1407026 Version 1 (Component)
detect the intensity of precA

BBa_I742134
BBa_I742134 Version 1 (Component)
Recombinase XerC of the XerCD complex.

BBa_K233318
BBa_K233318 Version 1 (Component)
A tester for Snowdrift 1.

BBa_K233319
BBa_K233319 Version 1 (Component)
A tester for Snowdrift 2.

subE
BBa_K2020000 Version 1 (Component)
subtilisin E gene

BBa_M31902
BBa_M31902 Version 1 (Component)
HpaI to BamHI section of M13K07 genome

BBa_K736004
BBa_K736004 Version 1 (Component)
Coding sequence for insulin chain A

myc-gene I
BBa_K627007 Version 1 (Component)
Fusion of c-myc-tag and gene III

BBa_K523003
BBa_K523003 Version 1 (Component)
malS for BioSandwich

BBa_K2020040
BBa_K2020040 Version 1 (Component)
Fluorescent reporter for measurement of incorporation of ncAA

BBa_K1431503
BBa_K1431503 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K1431501
BBa_K1431501 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_K1431502
BBa_K1431502 Version 1 (Component)
Upstream Activation Sequence(UAS) bind to the GAL4 protein and then activate gene transcription

BBa_I20242
BBa_I20242 Version 1 (Component)
AHL Induced expression of T7 RNAP

BBa_K1945005
BBa_K1945005 Version 1 (Component)
C-terminal fragment of T7 RNA Polymerase

BBa_I13558
BBa_I13558 Version 1 (Component)
Ptet-driven expression of GFP and RFP

BBa_K199028
BBa_K199028 Version 1 (Component)
CGGUC tRNA Suppressor (Produces Serine)

BBa_K098984
BBa_K098984 Version 1 (Component)
lac inducible mtrB (high expression of QPI)

BBa_K098983
BBa_K098983 Version 1 (Component)
lac inducible mtrB (low expression of QPI)

BBa_K1709004
BBa_K1709004 Version 1 (Component)
LuxR-E

BBa_K112217
BBa_K112217 Version 1 (Component)
{a~ihfA!} The ihf alpha gene ready to have rbs attached with stop codon, BBb format

BBa_K112223
BBa_K112223 Version 1 (Component)
{a~ihfB!} The ihf beta gene ready to have rbs attached and stop codon, BBb format

BBa_K112211
BBa_K112211 Version 1 (Component)
{a~int!} The integrase gene with rbs ready to be attached with stop codon, BBb format

BBa_K1431101
BBa_K1431101 Version 1 (Component)
TetOn-3G, an ideal controller of mammalian gene expression with TRE-3G promoter+PolyA

BBa_K624041
BBa_K624041 Version 1 (Component)
pT7-tetO+RBS+minC+mcherry

BBa_K624056
BBa_K624056 Version 1 (Component)
pYMB essentials + RBS(Pmsp3) + minC

BBa_K299808
BBa_K299808 Version 1 (Component)
minC under pT7 and B0034

BBa_K299807
BBa_K299807 Version 1 (Component)
minC under pT7 and B0032

BBa_K624060
BBa_K624060 Version 1 (Component)
pYMB essentials + RBS(trunc.) + minC

BBa_K105022
BBa_K105022 Version 1 (Component)
lex A - operator

BBa_K209018
BBa_K209018 Version 1 (Component)
Aar1 A-B part, cAR1 (Dictyostelium discoideum)

BBa_K606011
BBa_K606011 Version 1 (Component)
IPTG/Lactose inducible expression casette for B. Subtilis

BBa_K783032
BBa_K783032 Version 1 (Component)
This is a MoClo converted version of BBa_J23115

BBa_K783028
BBa_K783028 Version 1 (Component)
This is a MoClo converted version of BBa_J23110

BBa_K783024
BBa_K783024 Version 1 (Component)
This is a MoClo converted version of BBa_R0063

BBa_R4030
BBa_R4030 Version 1 (Component)
PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)