BBa_K861111BBa_K861111 Version 1 (Component)BcsB with RBS and terminator
BBa_K861121BBa_K861121 Version 1 (Component)BcsZ with RBS and terminator
BBa_K861131BBa_K861131 Version 1 (Component)BcsC with RBS and terminator
pINDELBBa_K551001 Version 1 (Component)plasmid of insertion and deletion
BBa_K906011BBa_K906011 Version 1 (Component)Spacer with MluI and SacII
BBa_K124003BBa_K124003 Version 1 (Component)Bacteriophage 21 Lysis Cassette S, R, and Rz (PVJ4)
BBa_K1861050BBa_K1861050 Version 1 (Component)Fusion protein of TALE1 and a SpyTag in an expression cassette
BBa_K1861060BBa_K1861060 Version 1 (Component)Fusion protein of TALE2 and a SpyTag in an expression cassette
BBa_K1861070BBa_K1861070 Version 1 (Component)Fusion protein of TALE3 and a SpyTag in an expression cassette
BBa_K749017BBa_K749017 Version 1 (Component)Formaldehyde monitoring and dehydrogenate device
BBa_K749022BBa_K749022 Version 1 (Component)Formaldehyde monitoring and dehydrogenate device
BBa_K749016BBa_K749016 Version 1 (Component)Formaldehyde monitoring and dehydrogenate device
BBa_K2047011BBa_K2047011 Version 1 (Component)Stem-loop with free energy of -51.4 kcal/mol, measured by Mfold
BBa_K2047010BBa_K2047010 Version 1 (Component)Stem loop with free energy of -38.8 kcal/mol measured by Mfold
BBa_K2047013BBa_K2047013 Version 1 (Component)Stem-loop with free energy of -10.0 kcal/mol measured by Mfold
BBa_K2047002BBa_K2047002 Version 1 (Component)Stem-loop with free energy of -14.9 kcal/mol measured by Mfold
BBa_K2047009BBa_K2047009 Version 1 (Component)Stem loop with free energy of -25.6 kcal/mol measured by Mfold
BBa_K112405BBa_K112405 Version 1 (Component)Promoter for CadA and CadB genes
BBa_K606063BBa_K606063 Version 1 (Component)T7 amber expression monitored by a linear system
BBa_K1155006BBa_K1155006 Version 1 (Component)Promoter of narK activated by FNR in anaerobic condition
BBa_K1678000BBa_K1678000 Version 1 (Component)Promoter from BBa_J23119 followed by 4 orthogonal LoxP sites
P(narG)BBa_K1155005 Version 1 (Component)Promoter of narG activated by FNR in anaerobic conditions
P(nirB)BBa_K1155004 Version 1 (Component)Promoter of nirB, activated by FNR in anaerobic conditions
Atrazine-RBBa_K1145003 Version 1 (Component)A ribosome switch which can be opened by atrazine.
TBYBBa_K897720 Version 1 (Component)Antisense FtsZ protected by paired termini structure under T7 promoter
cspBBBa_K525123 Version 1 (Component)S-layer cspB from Corynebacterium glutamicum with lipid anchor and PT7 and RBS
BBa_I7104BBa_I7104 Version 1 (Component)T7 promoter and sRBS
BBa_R00100BBa_R00100 Version 1 (Component)Tet promoter and sRBS
BBa_I739019BBa_I739019 Version 1 (Component)Composite of BBa_I739010 and BBa_I739011
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_R8237BBa_R8237 Version 1 (Component)Dedicated promoter and RBS
BBa_K806002BBa_K806002 Version 1 (Component)hda - regulator of DnaA that prevents premature reinitiation of DNA replication
BBa_K2119123BBa_K2119123 Version 1 (Component)Cellobiose response regulator (ClbR) is a Zn(II)2Cys6 transcriptional activator.
IndFimRecGBBa_K1077002 Version 1 (Component)Inducible fimE and hbiF recombinase generator (tet and lux).
BBa_J107052BBa_J107052 Version 1 (Component)adhB constitutive generator (driven by a strong promoter)
BBa_I13561BBa_I13561 Version 1 (Component)polycistronic mRFP1 and GFP
BBa_I719001BBa_I719001 Version 1 (Component)ech and fcs genes
BBa_K2116059BBa_K2116059 Version 1 (Component)and gate_bxb1(medium tag)
BBa_P100102BBa_P100102 Version 1 (Component)BAMT ORF and RBS
BBa_K1463701BBa_K1463701 Version 1 (Component)MotA and B0032 RBS
BBa_I719011BBa_I719011 Version 1 (Component)ech and fcs genes
BBa_G100101BBa_G100101 Version 1 (Component)SAMT ORF and RBS
BBa_K654998BBa_K654998 Version 1 (Component)tet HyBD and TolC
AD-CIB1BBa_K1592006 Version 1 (Component)GalAD-CIB1 Fusion for Yeast-Two-Hybrid
BBa_K1179029BBa_K1179029 Version 1 (Component)Cas9-VN155 (D10A and H840A mutant)
BBa_K1179028BBa_K1179028 Version 1 (Component)Cas9-VC155 (D10A and H840 mutant)
BBa_K1796201BBa_K1796201 Version 1 (Component)An unloaded sgRNA that contains BbsI cutting site, with a promoter and terminator.
BBa_K861023BBa_K861023 Version 1 (Component)S-FadE with a RBS and a terminator
BBa_K1758370BBa_K1758370 Version 1 (Component)BlcR, repressor of blcABC operon, with promoter and RBS