BBa_K799026
BBa_K799026 Version 1 (Component)
pSB1C3 yGG transcriptional terminator acceptor vector

BBa_K195203
BBa_K195203 Version 1 (Component)
RBS+Lpp-OmpA-linker+alpha-V integrin gene+terminator

BBa_J95039
BBa_J95039 Version 1 (Component)
plasmid vector for Rhodobacter sphaeroides

BBa_K1231003
BBa_K1231003 Version 1 (Component)
Asr-64-Lpp-RBS-GFP is a dual-state construct

BBa_J01121
BBa_J01121 Version 1 (Component)
Sacrificial OnRFP AmpKan Vector

BBa_J01120
BBa_J01120 Version 1 (Component)
Sacrafical OnRFP Kan vector

BBa_K112995
BBa_K112995 Version 1 (Component)
BBb1 assembly vector - C/A

BBa_K584008
BBa_K584008 Version 1 (Component)
Lambda cI and LuxR regulated hybrid promotor + RBS + MelA + RBS + AFP + term

BBa_J72117
BBa_J72117 Version 1 (Component)
BBb High copy entry vector, pBca1256

BBa_K1321362
BBa_K1321362 Version 1 (Component)
sfGFP fused to CBDcex driven by LacI

BBa_K581003
BBa_K581003 Version 1 (Component)
SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K549004
BBa_K549004 Version 1 (Component)
LacI promotor fused with the iron dependent regulator fur

BBa_I741016
BBa_I741016 Version 1 (Component)
Reverse Total XylR Transcriptional Regulator Left Facing (I741011 reverse complement

BBa_K106693
BBa_K106693 Version 1 (Component)
AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)

BBa_K1615108
BBa_K1615108 Version 1 (Component)
mRFP fused to CBDclos driven by LacI promoter

BBa_K1114400
BBa_K1114400 Version 1 (Component)
This is a MoClo level 0 destination vector.

BBa_K258003
BBa_K258003 Version 1 (Component)
Granulysin, a T Cell Product,Kills Bacteria by Altering Membrane Permeability

BBa_K395102
BBa_K395102 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395005:K121013)

BBa_K395103
BBa_K395103 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395006:K121013)

BBa_J58014
BBa_J58014 Version 1 (Component)
Promoter activated by OmpR-P with the reporter GFP

BBa_K290001
BBa_K290001 Version 1 (Component)
constitutive RhlR with bicistronic LuxI - GFP controlled by pRhl

BBa_J58015
BBa_J58015 Version 1 (Component)
Mutated promoter activated by OmpR-P with the reporter GFP

BBa_K1033204
BBa_K1033204 Version 1 (Component)
pSBLb4E15 E. coli and lactobacilli shuttle vector with erythromycin resistance

BBa_K300096
BBa_K300096 Version 1 (Component)
Double phasin and intein separed by a flexible protein domain linker

BBa_K563053
BBa_K563053 Version 1 (Component)
vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.

BBa_K802003
BBa_K802003 Version 1 (Component)
Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>

BBa_K831011
BBa_K831011 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K831012
BBa_K831012 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.