BBa_K315030
BBa_K315030 Version 1 (Component)
pTet + loxP forward + RBS + RFP + lox 5171 forward

BBa_M36292
BBa_M36292 Version 1 (Component)
Transcription Terminator (99% efficient)

BBa_J58011
BBa_J58011 Version 1 (Component)
Promoter which is activated by cI and CRP, using a transcription logic function type AND

BBa_K833014
BBa_K833014 Version 1 (Component)
constitutive promoter followed by a lox site with a bidirectional terminator and then a lox site.

LacIm
BBa_K092800 Version 1 (Component)
Coding sequence for LacI modified with a different rate of translation than LacI, RBS

BBa_I715002
BBa_I715002 Version 1 (Component)
pLac-lox-RBS-Tet (in pSB3K3)

BBa_K315012
BBa_K315012 Version 1 (Component)
LoxBri (F) Variant forward lox site with 3 base changes in the spacer region

BBa_S03729
BBa_S03729 Version 1 (Component)
RFP(reverse)-TT-lox-RBS-Tet

BBa_K1159117
BBa_K1159117 Version 1 (Component)
Red light triggered Kill Switch for plants translation unit (PhyB/PIF3 version)

BBa_K1159118
BBa_K1159118 Version 1 (Component)
Red light triggered Kill Switch for plants translation unit (PhyB/PIF6 version)

BBa_K1075018
BBa_K1075018 Version 1 (Component)
J23105-RBS32-SspB[Core]-LOV-ipaA-TT

BBa_K176249
BBa_K176249 Version 1 (Component)
Low input score automatic selector w/o lethal device(with reporter): PoPS->mRFP->CI-LVA+pCI->K176240

BBa_K1613012
BBa_K1613012 Version 1 (Component)
QsrR Binding Site inhibits the transcription and translation of Red Fluorescent Protein.

BBa_T6002
BBa_T6002 Version 1 (Component)
test translational unit

BBa_K212006
BBa_K212006 Version 1 (Component)
mRBP (modified ribose binding protein - now binds histamine)

BBa_K576012
BBa_K576012 Version 1 (Component)
J23101-RBS-GFP1-Lox-GFP2-Transcription Terminator

BBa_K1087016
BBa_K1087016 Version 1 (Component)
Ptet- reverse lox- reverse PO promoter- mRFP1

BBa_K1075019
BBa_K1075019 Version 1 (Component)
AraC-pBAD-RBS32-SspB[Core]-LOV-ipaA-TT

BBa_K785004
BBa_K785004 Version 1 (Component)
Lov-HTH (light sensor with Ptet repression) light->PtetO repressed

BBa_K223032
BBa_K223032 Version 1 (Component)
trp-operon + RBS + GFP + TT makes sure trp-operon functions

BBa_I711026
BBa_I711026 Version 1 (Component)
Amy is learning how to build a biobrick. Hor.

BBa_K576011
BBa_K576011 Version 1 (Component)
J23101-RBS-GFP1-IN1-Lox-IN2-GFP2-TT

BBa_K809108
BBa_K809108 Version 1 (Component)
Efficiency test of Q0255 Terminator

BBa_K1159120
BBa_K1159120 Version 1 (Component)
Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF6 version)

BBa_M36556
BBa_M36556 Version 1 (Component)
5' Bicistronic UTR (medium), does not include ATG start

BBa_K1159119
BBa_K1159119 Version 1 (Component)
Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF3 version)

BBa_K1991007
BBa_K1991007 Version 1 (Component)
Pcons-RBS-LO-BamHI

BBa_K315002
BBa_K315002 Version 1 (Component)
variant reverse lox site with 2 base changes in the spacer region (lox5171)

BBa_K1991010
BBa_K1991010 Version 1 (Component)
Pcons-RBS-LO-GFP-His

BBa_K1991005
BBa_K1991005 Version 1 (Component)
LO-AOX1-His (Lpp-OmpA-Alcohol Oxidase)

BBa_K290001
BBa_K290001 Version 1 (Component)
constitutive RhlR with bicistronic LuxI - GFP controlled by pRhl

BBa_M36291
BBa_M36291 Version 1 (Component)
Transcription terminator (apFAB391) (99% efficient)

BBa_K1991008
BBa_K1991008 Version 1 (Component)
Pcons-RBS-LO-AOX1-His

BBa_K1991009
BBa_K1991009 Version 1 (Component)
Pcons-RBS-LO-AOX2-His

BBa_K987001
BBa_K987001 Version 1 (Component)
This is a composite part which has the function to invert the temperature activation by the part: BB

BBa_K315005
BBa_K315005 Version 1 (Component)
variant reverse lox site with 3 base changes in the spacer region (loxm2 reverse)

loxBri (R)
BBa_K315006 Version 1 (Component)
LoxBri (R)variant reverse lox site with 3 base changes in the spacer region

BBa_K185053
BBa_K185053 Version 1 (Component)
Promoter116+RBS34+LacI+Double terminator+Plac+RBS31+RelE+RBS30+Lon protease+Double terminator

BBa_S03737
BBa_S03737 Version 1 (Component)
pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)

BBa_K1091002
BBa_K1091002 Version 1 (Component)
green fluorescent protein－slow

BBa_S03736
BBa_S03736 Version 1 (Component)
pLac-lox-RBS-Tet (in pSB1A2)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.