View

BBa_K185053

BBa_K185053 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K185053

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shikun Zhao

Date created: 2009-10-17 11:00:00

Date modified: 2015-05-08 01:11:07

Promoter116+RBS34+LacI+Double terminator+Plac+RBS31+RelE+RBS30+Lon protease+Double terminator

Back

Details

Types	DnaRegion
Roles	Device engineered_region
Sequences	BBa_K185053_sequence (Version 1)

Description

This device composes of promoter, RBS, LacI, Plac, RelE, Lon and so on, which can express relE and Lon protein. The protein expression is controled by lacI.

Notes

We wish the expression of RelE and Lon protease can be controled by LacI.

Source

Promoter, RBS, lacI, plac, double terminator are come from 2009 iGEM biobrick. RelE and Lon protease come from E coli genome DNA

Components

Access	Instance	Definition
public public public public public public public public public public public public	BBa_J23116 BBa_B0034 BBa_I732100 BBa_B0010 BBa_B0012 BBa_R0010 BBa_B0031 BBa_K185047 BBa_B0030 BBa_K185049 BBa_B0010 BBa_B0012	BBa_J23116 BBa_B0034 LacI BBa_B0010 BBa_B0012 LacI BBa_B0031 BBa_K185047 BBa_B0030 BBa_K185049 BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23116 BBa_B0034 BBa_I732100 BBa_B0010 BBa_B0012 BBa_R0010 BBa_B0031 BBa_K185047 BBa_B0030 BBa_K185049 BBa_B0010 BBa_B0012	1,35 44,55 62,1147 1156,1235 1244,1284 1293,1492 1501,1514 1521,1826 1835,1849 1856,4210 4219,4298 4307,4347	BBa_J23116 BBa_B0034 LacI BBa_B0010 BBa_B0012 LacI BBa_B0031 BBa_K185047 BBa_B0030 BBa_K185049 BBa_B0010 BBa_B0012

Other Properties

igem#experience	None
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K185053/1