BBa_K1453402
BBa_K1453402 Version 1 (Component)
ssDsbA-Lgt-Enzyme USB(BsmAI)-TAL USB-His tag

BBa_J70588
BBa_J70588 Version 1 (Component)
{15,18;13,10} BsaXI BioScaffold (reverse R0040-B0034-E1010-B0010-B0012)

IBMc360
IBMc360 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry(1-192)-G-M86(1-100)-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(101-154)-S-mCherry(193-236)-H6-T

IBMc378
IBMc378 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-17)-PI-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(18-154)-S-mCherry*(193-236)-H6-T

IBMc379
IBMc379 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-22)-LG-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(23-154)-S-mCherry*(193-236)-H6-T

IBMc380
IBMc380 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-39)-LD-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(40-154)-S-mCherry*(193-236)-H6-T

BBa_K077557
BBa_K077557 Version 1 (Component)
Part 1 of the Conways game of life system

BBa_J70660
BBa_J70660 Version 1 (Component)
J70577 cut with BsaXI and with 6his inserts (J70564-fi) added

BBa_J70556
BBa_J70556 Version 1 (Component)
BsaXI test circuit: J70555.J70554 or J70554.J70550.J70554

BBa_K180007
BBa_K180007 Version 1 (Component)
Game of Life - Secondary plasmid [tac pR, LuxI generator]

BBa_K363009
BBa_K363009 Version 1 (Component)
Mid1, votage and stretch gated calcium channel from S. cerevisiae

BBa_J70596
BBa_J70596 Version 1 (Component)
J70577 cut with BsaXI and with 6his inserts (J70564-fi) added

BBa_M41008
BBa_M41008 Version 1 (Component)
AND Gate Promoter

BBa_J70597
BBa_J70597 Version 1 (Component)
J70577 cut with BsaXI and with 6his inserts (J70564-fi) added

BBa_K2116004
BBa_K2116004 Version 1 (Component)
AND gate regulated by norR and esaR (one esabox)

BBa_K1127002
BBa_K1127002 Version 1 (Component)
IM9 and A3-Flg fusion for gold binding

BBa_K310014
BBa_K310014 Version 1 (Component)
GolB (gold-binding protein) with RBS and terminator

BBa_M50016
BBa_M50016 Version 1 (Component)
Promoter, RBS and GolS Activator for Gold Detection

BBa_K1127018
BBa_K1127018 Version 1 (Component)
QS and gold sensing part for chracterisation

BBa_J70605
BBa_J70605 Version 1 (Component)
J70590 cut with BsaXI and with GSGSGS inserts (J70556-fi,ri) added

BBa_K2033002
BBa_K2033002 Version 1 (Component)
isovaleryl-HSL, 3-methyl-N-[(3S)-2-oxooxolan-3-yl]butanamide Sender- BjaI

BBa_K2045000
BBa_K2045000 Version 1 (Component)
FhuA-GBP:Ferrichrome Iron outer membrane transporter-gold binding peptide

BBa_M50026
BBa_M50026 Version 1 (Component)
Gold Detection Device with Promoter RBS and GolS

BBa_K310010
BBa_K310010 Version 1 (Component)
GolS: Transcription factor that is activated upon binding gold

LacIm
BBa_K092800 Version 1 (Component)
Coding sequence for LacI modified with a different rate of translation than LacI, RBS

BBa_J70606
BBa_J70606 Version 1 (Component)
J70590 cut with BsaXI and with dead fusion inserts (J70556d-fi,ri) added

BBa_K2033004
BBa_K2033004 Version 1 (Component)
(3S)-3-[(2-oxo-3-phenylpropyl)amino]oxolan-2-one (3-phenyl-HSL) Sender- BraI

BBa_K180006
BBa_K180006 Version 1 (Component)
Game of Life - Primary plasmid (part 2) [lux pR, GFP and LacI generator]

BBa_J70604
BBa_J70604 Version 1 (Component)
J70589 cut with BsaXI and with rbs, 6his inserts (J70559-f1i,f2i,r2i,r2i) added

BBa_K228013
BBa_K228013 Version 1 (Component)
(pSal PO) OR Gate - GFP

BBa_J54141
BBa_J54141 Version 1 (Component)
AND GATE"AHL and oleate"

BBa_K2116006
BBa_K2116006 Version 1 (Component)
AND gate regulated by norR and esaR (three esaboxes)

BBa_K547000
BBa_K547000 Version 1 (Component)
ready-to-inject backbone for T3SS, SlrP taged, with Bsa I cloning site

BBa_J70573
BBa_J70573 Version 1 (Component)
R0010 with BsaXI site removed

BBa_K1363604
BBa_K1363604 Version 1 (Component)
key of no of RNA logic gates

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.