BBa_S03644BBa_S03644 Version 1 (Component)RFP<sub>rev</sub> : RBS<sub>rev</sub>
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_B2102BBa_B2102 Version 1 (Component)T7 RBS 0.4 + SapI (rev)
Cph8 geneBBa_K566026 Version 1 (Component)Cph8 gene for red-photoreceptor (constitutive expression)
dCas 9BBa_K1051800 Version 1 (Component)protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.
iGEM 2019 DistributioniGEM2019Distribution_collection Version 1 (Collection)Distribution of parts for the 2019 iGEM competition
iGEM 2018 DistributioniGEM2018Distribution_collection Version 1 (Collection)Distribution of parts for the 2018 iGEM competition
iGEM DistributionsiGEMDistributions_collection Version 1 (Collection)Part distributions for the iGEM competition
Bacillus subtilis Collectionbsu_collection Version 1 (Collection)This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.
iGEM Parts Registryigem_collection Version 1 (Collection)The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.
BBa_S03636BBa_S03636 Version 1 (Component)TetB-RBS<sub>rev</sub> : HixC
BBa_K879531BBa_K879531 Version 1 (Component)Red with promoter 5
BBa_K879431BBa_K879431 Version 1 (Component)Red with promoter 4
BBa_K218001BBa_K218001 Version 1 (Component)LuxOU from Vibrio harveyi
BBa_K575006BBa_K575006 Version 1 (Component)Continous expression of RhlR receptor protein(with RBS, B0030)
BBa_K863021BBa_K863021 Version 1 (Component)bhal laccase from Bacillus halodurans
oprFBBa_K1172501 Version 1 (Component)Outer membrane porin OprF from Pseudomonas fluorescens
Pveg-spoVGBBa_K143053 Version 1 (Component)Promoter Pveg and RBS spoVG for B. subtilis
BBa_K900000BBa_K900000 Version 1 (Component)PAmCherry (photoactivatable red fluorescent protein)
BBa_K1159304BBa_K1159304 Version 1 (Component)Signal Peptide of Ig Kappa chain from <i>Mus musculus</i> in RFC[25] N-part
ReBBBa_K411104 Version 1 (Component)RFP_split_B + Linker + eIF4A_split_B
BBa_M36224BBa_M36224 Version 1 (Component)Red Luciferase for E. coli
BBa_K1015017BBa_K1015017 Version 1 (Component)FRT+Ap(for RED system)
BBa_K239008BBa_K239008 Version 1 (Component)RBS from RpoS
rbsABO_32549 Version 1 (Component) BBa_K1895996BBa_K1895996 Version 1 (Component)Red light sensitive 'LDR'
RBSBBa_K1642004 Version 1 (Component)SD sequence of cpcB
phlFBBa_K1725040 Version 1 (Component)<i>phlF</i> encoding PhlF repressor from Pseudomonas protegens Pf-5
BBa_S03676BBa_S03676 Version 1 (Component)RFP<sub>rev</sub>-RBS<sub>rev</sub>-HixC : TetB-RBS<sub>rev</sub>-HixC
BBa_I11021BBa_I11021 Version 1 (Component)excisionase from E. coli phage lambda (removes prophage from host genome)
BBa_B2101BBa_B2101 Version 1 (Component)T7 RBS 0.3 + SapI (rev)
rpsSBO_29191 Version 1 (Component) BBa_I742120BBa_I742120 Version 1 (Component)Lycopene (red pigment) producing operon
BBa_K1613012BBa_K1613012 Version 1 (Component)QsrR Binding Site inhibits the transcription and translation of Red Fluorescent Protein.
BBa_K640004BBa_K640004 Version 1 (Component)Device for conjugation of plasmids from E. coli to pseudomonas
pBB_RonLBBa_K1232003 Version 1 (Component)Red light inducible sfGFP = PLambda-RBS-LacZ-T1-POmpC-CI-T1
BBa_K1172901BBa_K1172901 Version 1 (Component)Alanine racemase from ''E. coli''
SbpA | RedBBa_K525412 Version 1 (Component)Fusion Protein of S-Layer SbpA and NADP+ Oxidoreductase
SgsE | RedBBa_K525312 Version 1 (Component)Fusion Protein of S-Layer SgsE and Oxidoreductase
BBa_K079038BBa_K079038 Version 1 (Component)TetO-wt/4C5G - operator library member
BBa_K091232BBa_K091232 Version 1 (Component)LuxR producer and RFP(rev) + RBS(rev) + pLux (for)
REXBBa_K809401 Version 1 (Component)REX coding sequence
PvegBBa_K316001 Version 1 (Component)pVeg Constitutive promoter for Veg locus from B. subtilis
BBa_I20260BBa_I20260 Version 1 (Component)Measurement Kit Test of J23101
BBa_K1491020BBa_K1491020 Version 1 (Component)Constitutive Codon Optimized Killer Red
BBa_K863001BBa_K863001 Version 1 (Component)bpul laccase from Bacillus pumilus
BBa_K863006BBa_K863006 Version 1 (Component)ecol laccase from E. coli
BBa_K863011BBa_K863011 Version 1 (Component)tthl laccase from Thermus thermophilus
BBa_K784007BBa_K784007 Version 1 (Component)Spacer + RBS from theophylline riboswitch
BBa_S03596BBa_S03596 Version 1 (Component)AraCPC<sub>rev</sub>-TT : pBad-HixC-RBS-TetF-HixC-TT-RE