BBa_K1824008
BBa_K1824008 Version 1 (Component)
Consensus Promoter T7 + RNA Thermometer U6

BBa_K1824006
BBa_K1824006 Version 1 (Component)
Consensus Promoter T7 + RNA Thermometer A1

BBa_K313000
BBa_K313000 Version 1 (Component)
gfp generator with T7 promoter

T7rnap-G
BBa_I716104 Version 1 (Component)
T7rnap (T7 RNA Polymerase) - GTG Start Codon

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K2117006
BBa_K2117006 Version 1 (Component)
TEF1 promoter with YFP

BBa_K1228007
BBa_K1228007 Version 1 (Component)
38aa with Constitutive promoter veg

BBa_K1114208
BBa_K1114208 Version 1 (Component)
E0030_CD, YFP Reporter

9A1
BBa_K590057 Version 1 (Component)
RrD_Y21W-PSB1C3-High constitutive

1C2
BBa_K590054 Version 1 (Component)
RrD_N25M-PSB1C3-High constitutive

BBa_K1450000
BBa_K1450000 Version 1 (Component)
T7 DNA polymerase

BBa_K1758107
BBa_K1758107 Version 1 (Component)
SYFP2 under T7 promoter + RBS

BBa_K1321163
BBa_K1321163 Version 1 (Component)
NiBP driven by T7 promoter

BBa_I13602
BBa_I13602 Version 1 (Component)
Tet operator with CFP reporter (with LVA tag) [R/Tc+]

RGK
BBa_K1362434 Version 1 (Component)
RGK (extein)

BBa_K1673120
BBa_K1673120 Version 1 (Component)
Standard RFP with T7 Promoter

BBa_K1679022
BBa_K1679022 Version 1 (Component)
KIG, kcoil+intein+gfp

BBa_K1679027
BBa_K1679027 Version 1 (Component)
VMA, a thermosensitive intein

BBa_K165015
BBa_K165015 Version 1 (Component)
pADH1 yeast constituative promoter

BBa_K117008
BBa_K117008 Version 1 (Component)
pLsrA-YFP

BBa_K561000
BBa_K561000 Version 1 (Component)
Pvgb+YFP+tetR

BBa_K909014
BBa_K909014 Version 1 (Component)
Constitutive expression of pABA

BBa_J119063
BBa_J119063 Version 1 (Component)
pT7-RBS-GFP-TT-pLacI-RBS-T7 RNAP (mutant 2)

BBa_J119068
BBa_J119068 Version 1 (Component)
RFPrev-RBSrev-pT7rev-pLacI-RBS-T7 RNAP (2SAT mutant 1)

BBa_J119066
BBa_J119066 Version 1 (Component)
pT7-RBS-GFP-TT-pLacI-RBS-T7 RNAP (mutant 5)

BBa_J119064
BBa_J119064 Version 1 (Component)
pT7-RBS-GFP-TT-pLacI-RBS-T7 RNAP (mutant 3)

BBa_J119067
BBa_J119067 Version 1 (Component)
pT7-RBS-GFP-TT-pLacI-RBS-T7 RNAP (mutant 6)

BBa_J119065
BBa_J119065 Version 1 (Component)
pT7-RBS-GFP-TT-pLacI-RBS-T7 RNAP (mutant 4)

BBa_J119062
BBa_J119062 Version 1 (Component)
pT7-RBS-GFP-TT-pLacI-RBS-T7 RNAP (mutant 1)

BBa_K1956005
BBa_K1956005 Version 1 (Component)
RIBOSOFF 1 for lacI RNA under constitutive promoter

BBa_K1316017
BBa_K1316017 Version 1 (Component)
T7 lacO + mtrCAB with His tag

3xSWAG(N)
BBa_K1362900 Version 1 (Component)
N-terminal tripleSWAGtag (N-intein)

BBa_K1679037
BBa_K1679037 Version 1 (Component)
VMA Dead, an inactivated intein

BBa_K1362901
BBa_K1362901 Version 1 (Component)
C-terminal tripleSWAGtag (C-intein)

BBa_I716182
BBa_I716182 Version 1 (Component)
[T7][rbs][hemA from Rcap][rbs][hemB][rbs][hemC]

BBa_K606062
BBa_K606062 Version 1 (Component)
pVeg SopVg T7 RNA amber TT

BBa_K176103
BBa_K176103 Version 1 (Component)
constitutive promoter family member K176008 (pCon 0.15) with primer VR9 site