BBa_K564007
BBa_K564007 Version 1 (Component)
Regulatory device based on sRNA

BBa_K626006
BBa_K626006 Version 1 (Component)
Zinc Finger Protein 1. - Ecdysone Receptor LBD

BBa_K626007
BBa_K626007 Version 1 (Component)
Zinc Finger Protein 2. - Ecdysone Receptor LBD

BBa_K592021
BBa_K592021 Version 1 (Component)
PompC-tetR inverter-B0034-mCherry

Ptet-ChiXm
BBa_K564020 Version 1 (Component)
Mutated regulatory device based on sRNA.

BBa_K615001
BBa_K615001 Version 1 (Component)
Zinc finger-omega subunit fusion protein

BBa_K823040
BBa_K823040 Version 1 (Component)
Inverter: pBad-RyhB-pLac(R0011)-uof-lacZalpha

BBa_K887020
BBa_K887020 Version 1 (Component)
PBSII (zinc finger) + ilvC (enzyme)

BBa_K887021
BBa_K887021 Version 1 (Component)
Gli1(zinc finger)+kivd

BBa_K887060
BBa_K887060 Version 1 (Component)
B0034(RBS)+HivC(zinc finger)+ilvD+37

BBa_K887011
BBa_K887011 Version 1 (Component)
DNA program : specific DNA sequence which can be recognized by zinc fingers

BBa_K737025
BBa_K737025 Version 1 (Component)
PhoB inverter

BBa_K737028
BBa_K737028 Version 1 (Component)
Ugp inverter

BBa_K737031
BBa_K737031 Version 1 (Component)
Spot42 based small RNA1

BBa_K737032
BBa_K737032 Version 1 (Component)
Spot42 based small RNA2

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

BBa_K874001
BBa_K874001 Version 1 (Component)
Polydactyl Zinc Finger (PZF3838)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K177008
BBa_K177008 Version 1 (Component)
Phagosome localisation device (PhoQ based)

BBa_J69604
BBa_J69604 Version 1 (Component)
lacI Inverter Test Construct A

BBa_J69616
BBa_J69616 Version 1 (Component)
tetR Inverter Test Construct B

BBa_K249001
BBa_K249001 Version 1 (Component)
Arabinose Induced Inverter

BBa_K249014
BBa_K249014 Version 1 (Component)
C-terminal YFP --> Tet inverter

BBa_K249015
BBa_K249015 Version 1 (Component)
C-terminal CFP --> Tet inverter

BBa_K249017
BBa_K249017 Version 1 (Component)
N-terminal YFP --> Tet inverter

BBa_K249018
BBa_K249018 Version 1 (Component)
N-terminal CFP --> Tet inverter

BBa_K176107
BBa_K176107 Version 1 (Component)
CI Inverter + GFP Reporter: PoPS->cI-LVA+pCI->GFP

BBa_K176108
BBa_K176108 Version 1 (Component)
CI Inverter + GFP Reporter: VR10+pCon max->cI-LVA+pCI->GFP

BBa_K176109
BBa_K176109 Version 1 (Component)
CI Inverter + GFP Reporter: VR3+pCon 1.00->cI-LVA+pCI->GFP

BBa_K176110
BBa_K176110 Version 1 (Component)
CI Inverter + GFP Reporter: VR4+pCon 0.70->cI-LVA+pCI->GFP

BBa_K176111
BBa_K176111 Version 1 (Component)
CI Inverter + GFP Reporter: VR7+pCon 0.36->cI-LVA+pCI->GFP

BBa_K176112
BBa_K176112 Version 1 (Component)
CI Inverter + GFP Reporter: VR9+pCon 0.15->cI-LVA+pCI->GFP

BBa_K176113
BBa_K176113 Version 1 (Component)
CI Inverter + GFP Reporter: VR8+pCon 0.04->cI-LVA+pCI->GFP

BBa_K176114
BBa_K176114 Version 1 (Component)
CI Inverter + GFP Reporter: VR5+pCon 0.01->cI-LVA+pCI->GFP

BBa_K185033
BBa_K185033 Version 1 (Component)
An inverter of a special lactose operon system based on J23110-rbs34-lacI-dter-plac-rbs31

BBa_K173005
BBa_K173005 Version 1 (Component)
tetR logic inverter

BBa_K223059
BBa_K223059 Version 1 (Component)
mutTrp (5MT) Inverter with GFP Biobrick part

BBa_K223060
BBa_K223060 Version 1 (Component)
5MT Inverter-GFP Reporter and Lac Promoter

BBa_K364101
BBa_K364101 Version 1 (Component)
Zinc Finger Protein AAA

BBa_K364102
BBa_K364102 Version 1 (Component)
Zinc Finger Protein AAG

BBa_K364103
BBa_K364103 Version 1 (Component)
Zinc Finger Protein AAC

BBa_K364104
BBa_K364104 Version 1 (Component)
Zinc Finger Protein AAT

BBa_K364105
BBa_K364105 Version 1 (Component)
Zinc Finger Protein AGA

BBa_K364106
BBa_K364106 Version 1 (Component)
Zinc Finger Protein AGG

BBa_K364107
BBa_K364107 Version 1 (Component)
Zinc Finger Protein AGC

BBa_K364108
BBa_K364108 Version 1 (Component)
Zinc Finger Protein AGT

BBa_K364109
BBa_K364109 Version 1 (Component)
Zinc Finger Protein ACA

BBa_K364110
BBa_K364110 Version 1 (Component)
Zinc Finger Protein ACG

BBa_K364111
BBa_K364111 Version 1 (Component)
Zinc Finger Protein ACC

BBa_K364112
BBa_K364112 Version 1 (Component)
Zinc Finger Protein ACT