BBa_K1321096
BBa_K1321096 Version 1 (Component)
Linker-CBDcipA-Linker + Phytochelatin (PC) EC20

BBa_K411122
BBa_K411122 Version 1 (Component)
split-RFP-A+linker+split-eIF4A-A+J13002+terminator

BBa_K1260010
BBa_K1260010 Version 1 (Component)
RBS 0030+ membrane protein LGT+double terminter

BBa_K1714006
BBa_K1714006 Version 1 (Component)
pBad/araC-RBS B0034-Ag43 thanatin 3mer as alpha domain-double terminator

BBa_K1916011
BBa_K1916011 Version 1 (Component)
CBCR2 + intein-CBD tag

BBa_K1916022
BBa_K1916022 Version 1 (Component)
CBCR11 + intein-CBD tag

BBa_K1916005
BBa_K1916005 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916008
BBa_K1916008 Version 1 (Component)
Osc7112_5903g3 + intein-CBD tag

BBa_K1916010
BBa_K1916010 Version 1 (Component)
CBCR2 + intein-CBD tag

BBa_K1916023
BBa_K1916023 Version 1 (Component)
CBCR11 + intein-CBD tag

BBa_K1916017
BBa_K1916017 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916015
BBa_K1916015 Version 1 (Component)
CBCR4 + intein-CBD tag

BBa_K1916020
BBa_K1916020 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916019
BBa_K1916019 Version 1 (Component)
CBCR8 + intein-CBD tag

BBa_K1916009
BBa_K1916009 Version 1 (Component)
Osc7112_5903g3 + intein-CBD tag

BBa_K1916001
BBa_K1916001 Version 1 (Component)
NpF2164g5 + intein-CBD tag

BBa_K1916018
BBa_K1916018 Version 1 (Component)
CBCR8 + intein-CBD tag

BBa_K1916013
BBa_K1916013 Version 1 (Component)
CBCR3 + intein-CBD tag

BBa_K1916007
BBa_K1916007 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1916014
BBa_K1916014 Version 1 (Component)
CBCR4 + intein-CBD tag

BBa_K1916000
BBa_K1916000 Version 1 (Component)
NpF2164g5 + intein-CBD tag

BBa_K1916003
BBa_K1916003 Version 1 (Component)
Cha6605_4344g2 + intein-CBD tag

BBa_K1916006
BBa_K1916006 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1435000
BBa_K1435000 Version 1 (Component)
4-HT inducible intein

BBa_K1916002
BBa_K1916002 Version 1 (Component)
Cyan7427_1390g3 + intein-CBD tag

BBa_K1916021
BBa_K1916021 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916004
BBa_K1916004 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916016
BBa_K1916016 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916012
BBa_K1916012 Version 1 (Component)
CBCR3 + intein-CBD tag

BBa_K1861020
BBa_K1861020 Version 1 (Component)
Fusion protein of the TALE constant domain and a SpyTag in an expression cassette

BBa_J52039
BBa_J52039 Version 1 (Component)
CMV + Rluc-linker-PEST191

BBa_K863110
BBa_K863110 Version 1 (Component)
Cellulose binding Domain from Clostridium cellulovorans cellulose binding protein

BBa_K861173
BBa_K861173 Version 1 (Component)
mRFP generator controlled by PcstA (glucose-repressible promoter)

BBa_K2017006
BBa_K2017006 Version 1 (Component)
RSIAT-TEV linker + Luciferase

BBa_M45121
BBa_M45121 Version 1 (Component)
RBS and phasin

BBa_M45131
BBa_M45131 Version 1 (Component)
RBS and Phasin

BBa_M45994
BBa_M45994 Version 1 (Component)
RBS and phasin

BBa_K1485002
BBa_K1485002 Version 1 (Component)
A kind of GFP for eukaryotes with a linker (GS)x5

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K2169139
BBa_K2169139 Version 1 (Component)
GS7 Linker

BBa_K1365999
BBa_K1365999 Version 1 (Component)
Green Fluorescent protein including the RBS and Double Terminator

creD
BBa_J64905 Version 1 (Component)
creD inner membrane protein

H6-Linker
BBa_K1362435 Version 1 (Component)
His6 linker for λ-Lysozyme

BBa_K195203
BBa_K195203 Version 1 (Component)
RBS+Lpp-OmpA-linker+alpha-V integrin gene+terminator

BBa_K092300
BBa_K092300 Version 1 (Component)
RFP controlled by a TetR promoter

BBa_K364107
BBa_K364107 Version 1 (Component)
Zinc Finger Protein AGC