Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xenia Spencer-Milnes
Date created: 2014-10-16 11:00:00
Date modified: 2015-06-17 12:35:09
Linker-CBDcipA-Linker + Phytochelatin (PC) EC20
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1321096_sequence (Version 1) |
Description
Synthetic phytochelatin EC20 (metal binding peptide) fused C-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.
This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals (project page). Other fusion parts with this metal binding protein can be seen in the table below:
Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.
Notes
The linker sequences mean fusions should be fine either N or N-terminally to the CBDcipASource
Please see part pages for the composite parts| Access | Instance | Definition |
| public public public | BBa_K1321014 BBa_B0105 BBa_K1321005 | BBa_K1321014 Scar 25 BBa_K1321005 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1321014 BBa_B0105 BBa_K1321005 | 1,711 712,717 718,840 | BBa_K1321014 Scar 25 BBa_K1321005 |