BBa_J72182
BBa_J72182 Version 1 (Component)
repA expression cassette

BBa_J72183
BBa_J72183 Version 1 (Component)
GFP Expression cassette

BBa_J72184
BBa_J72184 Version 1 (Component)
RepA Expression Cassette

BBa_J72185
BBa_J72185 Version 1 (Component)
GFP expression cassette

BBa_J72186
BBa_J72186 Version 1 (Component)
TrimR, pir expression cassette for genomic integration

BBa_J72188
BBa_J72188 Version 1 (Component)
weak violacein expression cassette

BBa_J72189
BBa_J72189 Version 1 (Component)
strong violacein expression cassette

BBa_J72190
BBa_J72190 Version 1 (Component)
neuS expression cassette

BBa_J72191
BBa_J72191 Version 1 (Component)
wbbL expression cassette

BBa_J72192
BBa_J72192 Version 1 (Component)
manB and neuS expression cassette

BBa_J72193
BBa_J72193 Version 1 (Component)
manB expression cassette

BBa_J72194
BBa_J72194 Version 1 (Component)
neuS expression cassette

BBa_J72195
BBa_J72195 Version 1 (Component)
Barnase/Barstar expression cassette

BBa_J72199
BBa_J72199 Version 1 (Component)
wbbL neuS expression cassette

BBa_J72201
BBa_J72201 Version 1 (Component)
BamHI endonuclease expression cassette

BBa_K817018
BBa_K817018 Version 1 (Component)
gamma-delta resolvase cassette

BBa_K817015
BBa_K817015 Version 1 (Component)
srnBC toxin-antitoxin cassette

BBa_K812014
BBa_K812014 Version 1 (Component)
Auxin production device for expression in tadpole

BBa_K750000
BBa_K750000 Version 1 (Component)
pBADGLT:unstable gfp expression device activated by arabinose

BBa_K750001
BBa_K750001 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 1.0 strength)

BBa_K750002
BBa_K750002 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.6 strength)

BBa_K750003
BBa_K750003 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.3 strength)

BBa_K750004
BBa_K750004 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.07 strength)

BBa_K750005
BBa_K750005 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.01 strength)

BBa_K750006
BBa_K750006 Version 1 (Component)
LuxR expression device activated by arabinose

BBa_K737058
BBa_K737058 Version 1 (Component)
Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

BBa_K098992
BBa_K098992 Version 1 (Component)
mtrB, high expression

BBa_K098984
BBa_K098984 Version 1 (Component)
lac inducible mtrB (high expression of QPI)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_K098993
BBa_K098993 Version 1 (Component)
heat sensitive cI QPI with low promoter

BBa_K098990
BBa_K098990 Version 1 (Component)
mtrB, low expression

BBa_K098987
BBa_K098987 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and low promoter

BBa_K098985
BBa_K098985 Version 1 (Component)
lac QPI driven by low promoter

BBa_K098982
BBa_K098982 Version 1 (Component)
lac inducible system with low promoter and GFP reporter

BBa_K098983
BBa_K098983 Version 1 (Component)
lac inducible mtrB (low expression of QPI)

BBa_K098980
BBa_K098980 Version 1 (Component)
tet QPI driven by low promoter

BBa_K098010
BBa_K098010 Version 1 (Component)
HO-pcyA, low expression

BBa_K098011
BBa_K098011 Version 1 (Component)
E. coli ompR, expressed at low level

BBa_K124017
BBa_K124017 Version 1 (Component)
Bacteriophage Lysis Cassette S105, R, and Rz

BBa_K143077
BBa_K143077 Version 1 (Component)
P43-gsiB GFP expression construct

BBa_K143078
BBa_K143078 Version 1 (Component)
P43-spoVG GFP expression construct

BBa_K143079
BBa_K143079 Version 1 (Component)
Pveg-spoVG GFP expression construct

BBa_K143080
BBa_K143080 Version 1 (Component)
P43-gsiB RFP expression construct

BBa_K143081
BBa_K143081 Version 1 (Component)
P43-spoVG RFP expression construct

BBa_K143082
BBa_K143082 Version 1 (Component)
Pveg-spoVG RFP expression construct

pBSEP1
BBa_K090401 Version 1 (Component)
Gram-positive Shuttle Vector for Episomal Expression

BBa_K086001
BBa_K086001 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086002
BBa_K086002 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP