BBa_I13035
BBa_I13035 Version 1 (Component)
3OC₆HSL Receiver Device with Inducible Control of LuxR and a YFP Output device

BBa_J119316
BBa_J119316 Version 1 (Component)
Scaffold 2.0 for J-GGA (With the promoter between Junction A & B and the RBS in the junction B & C)

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_K1228001
BBa_K1228001 Version 1 (Component)
A fragment of loctoferrin

BBa_K1334028
BBa_K1334028 Version 1 (Component)
IPTG activated promoter similar to R0011

BBa_K1022129
BBa_K1022129 Version 1 (Component)
pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Magainin II:RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Ly

BBa_K1022128
BBa_K1022128 Version 1 (Component)
pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Signiferin: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Ly

BBa_K1022127
BBa_K1022127 Version 1 (Component)
pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: MaximinH5: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Lys

BBa_M34567
BBa_M34567 Version 1 (Component)
converts PoPS to foobob

BBa_K2044006
BBa_K2044006 Version 1 (Component)
Based on our project,<3,6> is the direct pathway from Site No.3 to Site No.6 in the map we design.

BBa_K2044004
BBa_K2044004 Version 1 (Component)
Based on our project, <2,4> is the direct pathway from Site No.2 to Site No.4 in the map we design.

BBa_K2044009
BBa_K2044009 Version 1 (Component)
Based on our project, <4,8> is the direct pathway from Site No.4 to Site No.8 in the map we design.

BBa_K2044014
BBa_K2044014 Version 1 (Component)
Based on our project, <1,4> is the direct pathway from Site No.1 to Site No.4 in the map we design.

BBa_K2044007
BBa_K2044007 Version 1 (Component)
Based on our project, <4,5> is the direct pathway from Site No.4 to Site No.5 in the map we design.

BBa_K2044013
BBa_K2044013 Version 1 (Component)
Based on our project,<7,8> is the direct pathway from Site No.7 to Site No.8 in the map we design.

BBa_K2044008
BBa_K2044008 Version 1 (Component)
Based on our project, <4,6> is the direct pathway from Site No.4 to Site No.6 in the map we design.

BBa_K2044003
BBa_K2044003 Version 1 (Component)
Based on our project, <2,3> is the direct pathway from Site No.2 to Site No.3 in the map we design.

BBa_K2044002
BBa_K2044002 Version 1 (Component)
Based on our project, <2,1> is the direct pathway from Site No.2 to Site No.1 in the map we design.

BBa_K2044005
BBa_K2044005 Version 1 (Component)
Based on our project, <2,6> is the direct pathway from Site No.2 to Site No.6 in the map we design.

BBa_K2044012
BBa_K2044012 Version 1 (Component)
Based on our project, <6,8> is the direct pathway from Site No.6 to Site No.8 in the map we design.

BBa_K2044011
BBa_K2044011 Version 1 (Component)
Based on our project,<6,4> is the direct pathway from Site No.6 to Site No.4 in the map we design.

BBa_K112995
BBa_K112995 Version 1 (Component)
BBb1 assembly vector - C/A

BBa_K594011
BBa_K594011 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL and ECFP reporter.

BBa_K1707028
BBa_K1707028 Version 1 (Component)
CFP-ssRA downstream of a cI regulated promoter

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K594014
BBa_K594014 Version 1 (Component)
A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.

BBa_J70524
BBa_J70524 Version 1 (Component)
hard codes sequence of J70514 into a part

BBa_J70523
BBa_J70523 Version 1 (Component)
hard codes sequence of J70518 into a part

BBa_J70521
BBa_J70521 Version 1 (Component)
hard codes sequence of J70516 into a part

BBa_J70522
BBa_J70522 Version 1 (Component)
hard codes sequence of J70517 into a part

BBa_J70520
BBa_J70520 Version 1 (Component)
hard codes sequence of J70515 into a part

BBa_K1113001
BBa_K1113001 Version 1 (Component)
pSB4K5 with a promoter and RBS

Prm+GFP
BBa_S03336 Version 1 (Component)
Prm+ with GFP as a reporter

BBa_K1616003
BBa_K1616003 Version 1 (Component)
VVD link to YC155 (YFP Cter split)

BBa_K1616004
BBa_K1616004 Version 1 (Component)
VVD linked to YN155 (YFP Nter split)

BBa_K1323019
BBa_K1323019 Version 1 (Component)
Hfq expression cassette under a xylose inducible promoter

BBa_K1321362
BBa_K1321362 Version 1 (Component)
sfGFP fused to CBDcex driven by LacI

BBa_K936012
BBa_K936012 Version 1 (Component)
Leader sequence that brings protein to periplasm

BBa_J58008
BBa_J58008 Version 1 (Component)
Periplasmic binding protein that docks a vanillin molecule

BBa_I733004
BBa_I733004 Version 1 (Component)
Produce LacZ alpha in response to AHL

BBa_K1363006
BBa_K1363006 Version 1 (Component)
an experimental part used to test the conjugation

BBa_K783043
BBa_K783043 Version 1 (Component)
This is a MoClo converted version of BBa_J23102

BBa_K783039
BBa_K783039 Version 1 (Component)
This is a MoClo converted version of BBa_J23101