BBa_K1956026BBa_K1956026 Version 1 (Component)Noise in Device measurement
BBa_K1956025BBa_K1956025 Version 1 (Component)Noise in Device measurement
BBa_K1956024BBa_K1956024 Version 1 (Component)Noise in Device measurement
BBa_K1956027BBa_K1956027 Version 1 (Component)Noise in Device measurement
BBa_K091135BBa_K091135 Version 1 (Component)Las Sender Testing Device
BBa_K239013BBa_K239013 Version 1 (Component)tetR consititutive YFP Device
BBa_I3510BBa_I3510 Version 1 (Component)Quorum receiver device (R0063.I0462.R0062)
BBa_K886002BBa_K886002 Version 1 (Component)Recombination Device composed by Cre-lox71
BBa_K944001BBa_K944001 Version 1 (Component)Transcriptional Regulator for Cyanide Inducible Promoter
BBa_K594011BBa_K594011 Version 1 (Component)A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL and ECFP reporter.
BBa_K594014BBa_K594014 Version 1 (Component)A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.
BBa_K1695049BBa_K1695049 Version 1 (Component)pL8-UV5 + Riboswitch Bacteriophage 21 Codon Optimized Lysis Cassette S R Rz
BBa_K779603BBa_K779603 Version 1 (Component)Hef1a-eYFP-4xFF1 MammoBlock Device
BBa_J92001BBa_J92001 Version 1 (Component)Lead Remover and Reporter Device
pTetR-LacIBBa_I763030 Version 1 (Component)LacI coding device regulated by pTetR
BBa_K1323019BBa_K1323019 Version 1 (Component)Hfq expression cassette under a xylose inducible promoter
BBa_K299029BBa_K299029 Version 1 (Component)RBS measurement device pT7 J61127 GFP
BBa_K2092002BBa_K2092002 Version 1 (Component)PalcA, improved alcR inducible promoter from A. nidulans
BBa_K590046BBa_K590046 Version 1 (Component)AAR-PSB3K3-Lac Inducible w/o LacI
BBa_I763003BBa_I763003 Version 1 (Component)GFP coding device switched on by IPTG
BBa_K638201BBa_K638201 Version 1 (Component)Arabinose inducible Poly-His Reflectin A1 generator
BBa_K1088010BBa_K1088010 Version 1 (Component)E. coli dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG inducib
BBa_K418001BBa_K418001 Version 1 (Component)From partsregistry.org IPTG inducible Lac promoter cassette
Prom/RBSBBa_K262000 Version 1 (Component)BBa_R0011 & BBa_B0034, IPTG-inducible promoter with Elowitz RBS.
BBa_K1088057BBa_K1088057 Version 1 (Component)T25 domain of bacterial two-hybrid system (IPTG inducible)
BBa_K1606010BBa_K1606010 Version 1 (Component)Kumamax enzyme that gluten updated m-cherry reporter device
BBa_K511905BBa_K511905 Version 1 (Component)Repressible rtTA3 Transactivator Generator (Hef1a-LacO-rtTA3) MammoBlock Device
BBa_K1341011BBa_K1341011 Version 1 (Component)OR LOGIC GATE IN Graph Theory (GFP OUTPUT DEVICE)
BBa_K1968009BBa_K1968009 Version 1 (Component)PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger
AraC_TEV-FBBa_K627008 Version 1 (Component)Fusion part of arabinose-inducible induction system and the TEV protease
BBa_K563053BBa_K563053 Version 1 (Component)vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.
Adapter BiBBa_K1807000 Version 1 (Component)Protein generator device suitable for blue-white screening and Gibson Assembly.
BBa_K137033BBa_K137033 Version 1 (Component)Device with GFP with (AC)21 repeat after start codon
pSBBs0KBBa_K823026 Version 1 (Component)pSB<sub>Bs</sub>0K-P<sub>spac</sub> (replicative Bacillus subtilis expression vector; IPTG inducible
pCMV-ECFP-BBa_I763023 Version 1 (Component)LacI coding device with ECFP as a reporter regulated by pCMV
BBa_J04431BBa_J04431 Version 1 (Component)GFP Coding Device with promoter, RBS, GFP with LVA tag, and Terminator
BBa_K1075003BBa_K1075003 Version 1 (Component)Promoter(const.)-AraC-Term-pBAD-RBS34 (Arabinose inducable promoter system)
BBa_K2123117BBa_K2123117 Version 1 (Component)Novel RFP device regulated by mercury: MerR (regulatory protein) + Stationary phase with mer operato
iGEM Parts Registryigem_collection Version 1 (Collection)The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.