BBa_K1916003
BBa_K1916003 Version 1 (Component)
Cha6605_4344g2 + intein-CBD tag

BBa_K1916006
BBa_K1916006 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1435000
BBa_K1435000 Version 1 (Component)
4-HT inducible intein

BBa_K1916002
BBa_K1916002 Version 1 (Component)
Cyan7427_1390g3 + intein-CBD tag

BBa_K1916021
BBa_K1916021 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916004
BBa_K1916004 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916016
BBa_K1916016 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916012
BBa_K1916012 Version 1 (Component)
CBCR3 + intein-CBD tag

BBa_K1159995
BBa_K1159995 Version 1 (Component)
10/25 N-Part Assembly Scar

BBa_J70628
BBa_J70628 Version 1 (Component)
RFC12 Sce VMA Intein Internal Domain

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1362106
BBa_K1362106 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] assembly construct with additional C-teminal insertion site

BBa_J23018
BBa_J23018 Version 1 (Component)
EN-Ptet-rbs-X-[RFP]-SNP "ORF Expresser"

H6-sfGFP(N
BBa_K1362471 Version 1 (Component)
split sfGFPn half

RGK
BBa_K1362434 Version 1 (Component)
RGK (extein)

BBa_K1362107
BBa_K1362107 Version 1 (Component)
RBS + NpuDnaE C-intein RFC[105] assembly construct with additional N-teminal insertion site

BBa_K899000
BBa_K899000 Version 1 (Component)
Sucrose sucrose fructosyltransferase (SST)

BBa_K165007
BBa_K165007 Version 1 (Component)
Gli-1 DNA-binding domain

BBa_K1679022
BBa_K1679022 Version 1 (Component)
KIG, kcoil+intein+gfp

BBa_K1679027
BBa_K1679027 Version 1 (Component)
VMA, a thermosensitive intein

BBa_K1679037
BBa_K1679037 Version 1 (Component)
VMA Dead, an inactivated intein

BBa_K1362901
BBa_K1362901 Version 1 (Component)
C-terminal tripleSWAGtag (C-intein)

BBa_K1491027
BBa_K1491027 Version 1 (Component)
T7 RNA Polymerase with Dead ER Intein

BBa_K1997000
BBa_K1997000 Version 1 (Component)
sHRP-N

BBa_K1362166
BBa_K1362166 Version 1 (Component)
RBS + Gp41-1 N-intein RFC[105] assembly construct with additional C-teminal insertion site

BBa_K1446002
BBa_K1446002 Version 1 (Component)
superfolder GFP with N-terminal pdu-microcompartment tag

BBa_K1362167
BBa_K1362167 Version 1 (Component)
RBS + Gp41-1 C-intein RFC[105] assembly construct with additional N-teminal insertion site