BBa_K1859012
BBa_K1859012 Version 1 (Component)
2*GSGSGS linker+ the 5??side of the intron[BBa_K1332003]

BBa_K1859010
BBa_K1859010 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GSGSGS*3 linker

BBa_K1859009
BBa_K1859009 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GGSGGS*3 linker

BBa_K1859014
BBa_K1859014 Version 1 (Component)
3*GSGSGS linker+ the 5??side of the intron[BBa_K1332003]

BBa_K1859007
BBa_K1859007 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GGSGGS*2 linker

BBa_K1859011
BBa_K1859011 Version 1 (Component)
2*GGSGGS linker+ the 5??side of the intron[BBa_K1332003]

BBa_K1859008
BBa_K1859008 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GSGSGS*2 linker

BBa_K1859013
BBa_K1859013 Version 1 (Component)
3*GGSGGS linker+ the 5??side of the intron[BBa_K1332003]

CircNpu
BBa_K1362000 Version 1 (Component)
NpuDnaE intein RFC[105] circularization construct

BBa_J64600
BBa_J64600 Version 1 (Component)
lac operon

BBa_K1965011
BBa_K1965011 Version 1 (Component)
P5:cLuc

BBa_I713030
BBa_I713030 Version 1 (Component)
pACYC-Lux

BBa_K1965005
BBa_K1965005 Version 1 (Component)
P3:cLuc

BBa_K1965012
BBa_K1965012 Version 1 (Component)
P7:cLuc

BBa_K1997003
BBa_K1997003 Version 1 (Component)
sLuc-C

BBa_K1997002
BBa_K1997002 Version 1 (Component)
sLuc-N

BBa_K1965013
BBa_K1965013 Version 1 (Component)
P9:cLuc

BBa_K831009
BBa_K831009 Version 1 (Component)
Inducible HipB antitoxin under the control of lac promoter

BBa_K266003
BBa_K266003 Version 1 (Component)
POPS -> Lac Inverter -> LasR

BBa_K1903019
BBa_K1903019 Version 1 (Component)
Intein N SSp DnaB S1

BBa_K1486018
BBa_K1486018 Version 1 (Component)
Arabinose promoter + fLuc[1] + fLuc[2]

BBa_K1486021
BBa_K1486021 Version 1 (Component)
Arabinose promoter + rLuc[1] + rLuc[2]

KaiABC_exp
BBa_J36937 Version 1 (Component)
Lac+RBS+KaiA+Lac+RBS+KaiC+Lac+RBS+KaiB

BBa_K389050
BBa_K389050 Version 1 (Component)
Tightly controlled lac operon

BBa_J176061
BBa_J176061 Version 1 (Component)
luc miR sensor 9 (R/CMV-T/CMV)

BBa_J176058
BBa_J176058 Version 1 (Component)
luc miR sensor 6 (R/HPK-T/CMV)

BBa_J176056
BBa_J176056 Version 1 (Component)
luc miR sensor 4 (R/HPK-T/Ubc)

BBa_J176053
BBa_J176053 Version 1 (Component)
luc miR sensor 1 (R/Ubc-T/Ubc)

BBa_J176057
BBa_J176057 Version 1 (Component)
luc miR sensor 5 (R/HPK-T/HPK)

BBa_J176059
BBa_J176059 Version 1 (Component)
luc miR sensor 7 (R/CMV-T/Ubc)

BBa_J176054
BBa_J176054 Version 1 (Component)
luc miR sensor 2 (R/Ubc-T/HPK)

BBa_J176055
BBa_J176055 Version 1 (Component)
luc miR sensor 3 (R/Ubc-T/CMV)

SspDnaB(C)
BBa_K1362409 Version 1 (Component)
SspDnaB C-Intein cloning piece

BBa_K1222983
BBa_K1222983 Version 1 (Component)
Lac operator+CamR antisense 2/pSB1C3

BBa_K1856005
BBa_K1856005 Version 1 (Component)
lac-citrine

pLac
BBa_K1065107 Version 1 (Component)
Lac promoter

pSB_LIC
BBa_K155000 Version 1 (Component)
LIC plasmid

LacA
BBa_K366000 Version 1 (Component)
lac A

BBa_K1725223
BBa_K1725223 Version 1 (Component)
Lux ABCDE

1Gate-NpuD
BBa_K1362100 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] assembly construct (with His6)

BBa_I13606
BBa_I13606 Version 1 (Component)
Reporter Construct (Ty+Lc+)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

1GateNpuE
BBa_K1362101 Version 1 (Component)
RBS+NpuDnaE C-intein RFC[105] assembly construct (with His6)

BBa_I13976
BBa_I13976 Version 1 (Component)
intermediate for lac relaxation oscillator

T7/lac pro
BBa_K1362463 Version 1 (Component)
strong T7 promoter + LacO Regulator

BBa_K2023008
BBa_K2023008 Version 1 (Component)
Gluc-term

KaiBKaiC_e
BBa_J36336 Version 1 (Component)
Lac+RBS+KaiB+Lac+RBS+KaiC

BBa_K1051974
BBa_K1051974 Version 1 (Component)
clb6 P + D-box + GFP + MER2 Intron + TOM40 + T

SV40+Rluc
BBa_K1722013 Version 1 (Component)
SV40+Rluc Composite

BBa_J52018
BBa_J52018 Version 1 (Component)
NFkB + Rluc