BBa_K1903005
BBa_K1903005 Version 1 (Component)
Split TEV N-IntN DnaE

BBa_K1903007
BBa_K1903007 Version 1 (Component)
IntC DnaE-Split TEV

BBa_K1903006
BBa_K1903006 Version 1 (Component)
Split TEV N-IntN DnaE Device

BBa_K1903008
BBa_K1903008 Version 1 (Component)
Split TEV-IntC DnaE Device

BBa_K1903013
BBa_K1903013 Version 1 (Component)
Plac-RBS-Split TEV-IntN DnaE + PA1-RBS + Split TEV-IntC DnaE

BBa_K1945014
BBa_K1945014 Version 1 (Component)
Reverse complement of N-terminal fragment of split T7 RNA polymerase

BBa_K1945004
BBa_K1945004 Version 1 (Component)
N-terminal fragment of split T7 RNA polymerase

BBa_K411121
BBa_K411121 Version 1 (Component)
split_GFP_A+linker+split_eIF4A_A + terminator

BBa_K644002
BBa_K644002 Version 1 (Component)
ZipRR Heterodimerizing Leucine Zipper

rLuc
BBa_K518001 Version 1 (Component)
RBS + renilla luciferase + d.terminator

BBa_K588100
BBa_K588100 Version 1 (Component)
N-terminal leucine zipper

BBa_K738007
BBa_K738007 Version 1 (Component)
FB, Split GFP C-terminal domain

BBa_J72203
BBa_J72203 Version 1 (Component)
kanamycin resistant split R6K vector

BBa_J72204
BBa_J72204 Version 1 (Component)
ampicillin resistant split R6K vector w/ O16 HAs and FRT-Kan-FRT

BBa_J72205
BBa_J72205 Version 1 (Component)
ampicillin resistant split ColE2 vector

BBa_J72206
BBa_J72206 Version 1 (Component)
ampicillin resistant split R6K vector

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K157005
BBa_K157005 Version 1 (Component)
Split-Cerulean-cCFP

BBa_K157006
BBa_K157006 Version 1 (Component)
Split-Cerulean-nCFP

BBa_K157007
BBa_K157007 Version 1 (Component)
Split-Venus-cYFP

BBa_K157008
BBa_K157008 Version 1 (Component)
Split-Venus-nYFP

linker
BBa_K157009 Version 1 (Component)
Split fluorophore linker; Freiburg standard

Tm-Split-L
BBa_K157020 Version 1 (Component)
Tm-Split-Luciferase1/2

Tm-Splitlu
BBa_K157021 Version 1 (Component)
Tm-Splitluciferase2/2

SP-AntiNIP
BBa_K157030 Version 1 (Component)
SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2

SP-AntiNIP
BBa_K157031 Version 1 (Component)
SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2

SP-Lipocal
BBa_K157038 Version 1 (Component)
SP-Lipocalin-GGGSlinker-Tm-Splitluciferase2/2

SP-Lipocal
BBa_K157039 Version 1 (Component)
SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2

BBa_M10049
BBa_M10049 Version 1 (Component)
{˂GS5-IILK˃} - Leucine Zipper Peptide IILK

BBa_K243021
BBa_K243021 Version 1 (Component)
DsbA-His-DigA-Split Linker-Fok_a

BBa_K243022
BBa_K243022 Version 1 (Component)
DsbA-Strep-DigA-Split Linker-Fok_a

BBa_K243028
BBa_K243028 Version 1 (Component)
FOS-Split Linker-Fok_a

BBa_K243036
BBa_K243036 Version 1 (Component)
His-Dig-Split Linker-Fok_a