BBa_K299112
BBa_K299112 Version 1 (Component)
[promoter].[TxbaIG].[J61100RBS].[scarA=T].[RFP]

BBa_K1893034
BBa_K1893034 Version 1 (Component)
Scar

BBa_K1173501
BBa_K1173501 Version 1 (Component)
CcaS, CcaR, PcpcG2, cI(lambda), magenta

BBa_K861178
BBa_K861178 Version 1 (Component)
Pcar efficacy testing device with CRP overexpressed

BBa_K299110
BBa_K299110 Version 1 (Component)
[promoter].[TxbaIG].[J61100 RBS].[scarA=T].[YFP]

BBa_K299116
BBa_K299116 Version 1 (Component)
[promoter].[TxbaIG].[J61100 RBS].[scarA=T].[mCherryNLS]

BBa_K299115
BBa_K299115 Version 1 (Component)
[promoter].[TxbaIG].[J61100 RBS].[scarA=T].[SBFP]

BBa_K299113
BBa_K299113 Version 1 (Component)
[promoter].[TxbaIG].[J61100 RBS].[scarA=T].[mCherry]

BBa_K299117
BBa_K299117 Version 1 (Component)
[promoter].[TxbaIG].[J61100 RBS].[scarA=T].[mBanana]

BBa_K299111
BBa_K299111 Version 1 (Component)
[promoter].[TxbaIG].[J61100 RBS].[scarA=T].[CFP]

BBa_K299114
BBa_K299114 Version 1 (Component)
[promoter].[TxbaIG].[J61100 RBS].[scarA=T].[mOrrange]

BBa_K259008
BBa_K259008 Version 1 (Component)
BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker

BBa_K259009
BBa_K259009 Version 1 (Component)
BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker

BBa_K299801
BBa_K299801 Version 1 (Component)
standard biobrick scar (if next sequence does NOT start with ATG)

BBa_K1968021
BBa_K1968021 Version 1 (Component)
Synechocystis Sca6-2 Promoter with RBS* (LacI repressible) Phytobrick

BioScaffol
BBa_K259004 Version 1 (Component)
BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker

BioScaffol
BBa_K259002 Version 1 (Component)
BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker

BBa_K1833996
BBa_K1833996 Version 1 (Component)
Oligonucleotide synthesis scar

BBa_K902062
BBa_K902062 Version 1 (Component)
CAR (<i>Nocardia</i> carboxylic acid reductase)

BBa_K1897038
BBa_K1897038 Version 1 (Component)
Scar site generated by 3A Assembly

BBa_K1583054
BBa_K1583054 Version 1 (Component)
Non-coding scar site in between RBS and gene

BBa_K2055011
BBa_K2055011 Version 1 (Component)
8 bp Scar

BBa_K2055008
BBa_K2055008 Version 1 (Component)
6 bp Scar

BBa_K1232000
BBa_K1232000 Version 1 (Component)
Green light inducible sfGFP = [PCpcG2-RBS-sfGFP-CcaS](rev)-CcaR

BBa_K1897039
BBa_K1897039 Version 1 (Component)
Scar site generated by 3A Assembly

BBa_K1159991
BBa_K1159991 Version 1 (Component)
10/10 NC Assembly Scar

BBa_K299317
BBa_K299317 Version 1 (Component)
J23100[promoter].K299107[TxbaIG].J61117[RBS] No b-brick scars between

BBa_K299307
BBa_K299307 Version 1 (Component)
J23100[promoter].K299107[TxbaIG].J61107[RBS] No b-brick scars between

BBa_K299201
BBa_K299201 Version 1 (Component)
J23100[promoter].K299107[TxbaIG].J61101[RBS] **no b-brick scars between**

BBa_K299301
BBa_K299301 Version 1 (Component)
J23100[promoter].K299107[TxbaIG].J61101[RBS] **no b-brick scars between**

BBa_K299300
BBa_K299300 Version 1 (Component)
J23100[promoter].K299107[TxbaIG].J61100[RBS] No b-brick scars between

BBa_K299327
BBa_K299327 Version 1 (Component)
J23100[promoter].K299107[TxbaIG].J61127[RBS] No b-brick scars between

BBa_K874300
BBa_K874300 Version 1 (Component)
IPTG inducible expression of M.ScaI methyltansferase (IPTG -> M.ScaI) (ScaI silently mutated out)

BBa_K299800
BBa_K299800 Version 1 (Component)
standard biobrick scar (if next part starts with ATG)

BBa_K1159992
BBa_K1159992 Version 1 (Component)
10/10 C Assembly Scar

BBa_K1920000
BBa_K1920000 Version 1 (Component)
Pcar-RBS-RFP-TT

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.