BBa_K750000BBa_K750000 Version 1 (Component)pBADGLT:unstable gfp expression device activated by arabinose
BBa_K750001BBa_K750001 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 1.0 strength)
BBa_K750002BBa_K750002 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.6 strength)
BBa_K750003BBa_K750003 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.3 strength)
BBa_K750004BBa_K750004 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.07 strength)
BBa_K750005BBa_K750005 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.01 strength)
BBa_K750006BBa_K750006 Version 1 (Component)LuxR expression device activated by arabinose
BBa_K737058BBa_K737058 Version 1 (Component)Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose
BBa_J72218BBa_J72218 Version 1 (Component)kanamycin resistant p15A vector
BBa_K750008BBa_K750008 Version 1 (Component)Quorum sensing system based on LuxI and LuxR to control the expression of parts behind
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K098992BBa_K098992 Version 1 (Component)mtrB, high expression
BBa_K098990BBa_K098990 Version 1 (Component)mtrB, low expression
BBa_K098984BBa_K098984 Version 1 (Component)lac inducible mtrB (high expression of QPI)
BBa_K098983BBa_K098983 Version 1 (Component)lac inducible mtrB (low expression of QPI)
EukaryoticBBa_K157040 Version 1 (Component)Eukaryotic terminator vector (very similar to pSB1AK8)
TransfectiBBa_K157041 Version 1 (Component)Transfection vector (similar to pSB1AK8)
BBa_K098010BBa_K098010 Version 1 (Component)HO-pcyA, low expression
BBa_K106342BBa_K106342 Version 1 (Component)AB shuttle vector
BBa_K106343BBa_K106343 Version 1 (Component)BD shuttle vector
BBa_K143077BBa_K143077 Version 1 (Component)P43-gsiB GFP expression construct
BBa_K143078BBa_K143078 Version 1 (Component)P43-spoVG GFP expression construct
BBa_K143079BBa_K143079 Version 1 (Component)Pveg-spoVG GFP expression construct
BBa_K143080BBa_K143080 Version 1 (Component)P43-gsiB RFP expression construct
BBa_K143081BBa_K143081 Version 1 (Component)P43-spoVG RFP expression construct
BBa_K143082BBa_K143082 Version 1 (Component)Pveg-spoVG RFP expression construct
pBSEP1BBa_K090401 Version 1 (Component)Gram-positive Shuttle Vector for Episomal Expression
BBa_K146000BBa_K146000 Version 1 (Component)pCR2.1 PCR product cloning vector
BBa_K106672BBa_K106672 Version 1 (Component)AarI AD acceptor vector (pRS305, Gal1P, Adh1t)
BBa_K106693BBa_K106693 Version 1 (Component)AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)
BBa_K106697BBa_K106697 Version 1 (Component)AarI AD acceptor vector (pRS315, Cyc1P, Adh1t-8XLexA Ops)
BBa_K142047BBa_K142047 Version 1 (Component)tet-controlled LacI generator with constitutive TetR expression cassette
BBa_K142024BBa_K142024 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197A) and TetR expression cassette
BBa_K142025BBa_K142025 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197F) and TetR expression cassette
BBa_K142026BBa_K142026 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (T276A) and TetR expression cassette
BBa_K142027BBa_K142027 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (T276F) and TetR expression cassette
BBa_K142028BBa_K142028 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276A)/TetR expression cassette
BBa_K142029BBa_K142029 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276F)/TetR expression cassette
BBa_K142030BBa_K142030 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276A)/TetR expression cassette
BBa_K142031BBa_K142031 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276F)/TetR expression cassette
BBa_K106698BBa_K106698 Version 1 (Component)AarI AD acceptor vector (pRS315, 8X LexAOps Fig1P, Adh1t)
BBa_K142073BBa_K142073 Version 1 (Component)lac-controlled GFP generator with constitutive lacI expression cassette
BBa_K142074BBa_K142074 Version 1 (Component)lac-controlled GFP-LVA generator with constitutive lacI expression cassette
BBa_K142075BBa_K142075 Version 1 (Component)lac-controlled CFP generator with constitutive lacI expression cassette
BBa_K142076BBa_K142076 Version 1 (Component)lac-controlled RFP generator with constitutive lacI expression cassette
BBa_K142048BBa_K142048 Version 1 (Component)promotorless lacI expression cassette followed by constitutively active TetR generator
BBa_K146001BBa_K146001 Version 1 (Component)AOX1 ethanol sensitive promoter cloned into RFP vector BBa_J61002
BBa_K106701BBa_K106701 Version 1 (Component)pCR2.1 TOPO Vector (to contain AarI inserts)
BBa_K165061BBa_K165061 Version 1 (Component)pRS304* yeast shuttle vector, TRP1 selection
BBa_K090402BBa_K090402 Version 1 (Component)B. subtilis Episomal Vector with Constitutive GFP