miniABBa_K877000 Version 1 (Component)Mini CipA coding region
BBa_K1733103BBa_K1733103 Version 1 (Component)Crisper/spCas9- with NLS
BBa_K1733099BBa_K1733099 Version 1 (Component)Crisper/spCas9- with NLS
BBa_K360041BBa_K360041 Version 1 (Component)Minimum Blue Light Receptor Promoter
BBa_K1559010BBa_K1559010 Version 1 (Component)RFP-Targeting protospacer for CRISPR/Cas9 system
BBa_K578001BBa_K578001 Version 1 (Component)E. coli CRISPR I leader sequence
BBa_K786032BBa_K786032 Version 1 (Component)cas3 protein in E.coli's CRISPR system
BBa_K512002BBa_K512002 Version 1 (Component)K12 CRISPR with GFP target spacer
BBa_J11031BBa_J11031 Version 1 (Component)Bacterial Whiteboard by Hannah (w/ Minimal Available Parts)
BBa_K1773000BBa_K1773000 Version 1 (Component)I-F type CRISPR-Cas Cascade complex
BBa_K1559006BBa_K1559006 Version 1 (Component)CRISPR/Cas9 system with pBAD inducible promoter
BBa_K1559007BBa_K1559007 Version 1 (Component)CRISPR/Cas9 system with pLac inducible promoter
BBa_K1559008BBa_K1559008 Version 1 (Component)CRISPR/Cas9 system with pRha inducible promoter
BBa_J100090BBa_J100090 Version 1 (Component)CRISPR sequence with GFP and AmpR targets
BBa_K578004BBa_K578004 Version 1 (Component)Bacillus halodurans C-125 CRISPR leader sequence
BBa_K1773002BBa_K1773002 Version 1 (Component)I-F type CRISPR-Cas Cas3 protein
BBa_J100085BBa_J100085 Version 1 (Component)short CRISPR sequence with GFP target spacer
BBa_K1773001BBa_K1773001 Version 1 (Component)I-F type CRISPR-Cas Cascade complex
BBa_K2060000BBa_K2060000 Version 1 (Component)CRISPR-Cas9 guide RNA targeting to 16S RNA
BBa_K1160000BBa_K1160000 Version 1 (Component)coding sequence of Cas9 from CRISPR system type II
BBa_K1559005BBa_K1559005 Version 1 (Component)CRISPR/Cas9 system with Anderson low-expression constitutive promoter
BBa_K1559003BBa_K1559003 Version 1 (Component)CRISPR/Cas9 system with Anderson high-expression constitutive promoter
BBa_K1559004BBa_K1559004 Version 1 (Component)CRISPR/Cas9 system with Anderson medium-expression constitutive promoter
CspRBO_9919 Version 1 (Component) cspRBO_32196 Version 1 (Component) BBa_K1774000BBa_K1774000 Version 1 (Component)CRISPR-Cas9 contaiment device repressed by arabinose and tryptophan
BBa_K2082251BBa_K2082251 Version 1 (Component)Sequence for rpoZ Knock-Out in E. coli through CRISPR/Cas9
BBa_K2087000BBa_K2087000 Version 1 (Component)CRISPR/Cas9 sgRNA Targeting Locus 618 on the DD96 Human Gene
BBa_K2087002BBa_K2087002 Version 1 (Component)CRISPR/Cas9 sgRNA Targeting Locus 401 on the DD96 Human Gene
BBa_K2116000BBa_K2116000 Version 1 (Component)RBS designed to achieve maximal expression of EsaR
BBa_K582000BBa_K582000 Version 1 (Component)500bp of Spacer/Repeat Region of the CRISPR genome present in Strep. thermophilus LMD9 strain
BBa_I714003BBa_I714003 Version 1 (Component)minimum oriT of plasmid R64 ( origin of transfer, R64 is IncI-1 )
BBa_K1051900BBa_K1051900 Version 1 (Component)G1 phase magic with cell synchronization device and CRISPRi induced alternative splicing device.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.