Lux pL/R
BBa_I13200 Version 1 (Component)
Natural LuxR/HSL Reg Promoter (Lux pL+Lux pR)

BBa_J119139
BBa_J119139 Version 1 (Component)
Device for Testing New Promoters via Golden Gate Assembly

BBa_J100104
BBa_J100104 Version 1 (Component)
For Testing New Promoters via Golden Gate Assembly v2

BBa_J119138
BBa_J119138 Version 1 (Component)
Device for Testing New Promoters via Golden Gate Assembly

BBa_J36852
BBa_J36852 Version 1 (Component)
Streptavidin, single-chain dimer (no start codon)

BBa_K208037
BBa_K208037 Version 1 (Component)
Lambda cL Reg. Promoter/RBS/GeneIII/PhaP/Terminator

BBa_K208040
BBa_K208040 Version 1 (Component)
Lambda cL Reg. Promoter/RBS/PelB/PhaP/Terminator

BBa_I714034
BBa_I714034 Version 1 (Component)
taRNA-key3-var1 (a new "key" designed by our team )

BBa_K1452000
BBa_K1452000 Version 1 (Component)
Pos/neg selection (Bacillus)

BBa_J119136
BBa_J119136 Version 1 (Component)
Device for Testing New Promoters via Golden Gate Assembly

BBa_J119141
BBa_J119141 Version 1 (Component)
Device for Testing New Promoters via BsgI Golden Gate Assembly

BBa_K563010
BBa_K563010 Version 1 (Component)
Tor2 gene from the genome of the S. cerevisiae, central protein in TOR(target of rapamycin) pathway

BBa_J119313
BBa_J119313 Version 1 (Component)
pClone Blue - Device for Testing New Promoters via Golden Gate Assembly

BBa_J119137
BBa_J119137 Version 1 (Component)
pClone Red - Device for Testing New Promoters via Golden Gate Assembly

BBa_K077041
BBa_K077041 Version 1 (Component)
AiiA and cII under control of plac promotor

BBa_K1607010
BBa_K1607010 Version 1 (Component)
The coding sequence of the SCFV of anti-p185her2/neu antibody chA21

BBa_K1228009
BBa_K1228009 Version 1 (Component)
38aa and 25aa fusion peptide with T1 terminator and Constitutive promoter veg

BBa_I718001
BBa_I718001 Version 1 (Component)
ech My new test generator part Feruloyl CoA hydratase for vanilin

BBa_K1124123
BBa_K1124123 Version 1 (Component)
inverse PCR template for creating new sRNA (plambda-micC sRNA scaffold-terminator)

pBAD-GFP
BBa_K845000 Version 1 (Component)
iGEM 2012 Grenoble Team proposes a new design and application to the pBAD promoter(paraBAD).

BBa_K1613016
BBa_K1613016 Version 1 (Component)
It is a new part which can detect cadmium ions in liquid.

BBa_K1613015
BBa_K1613015 Version 1 (Component)
It is a new part which can detect cadmium ions in liquid.

BBa_K1613014
BBa_K1613014 Version 1 (Component)
It is a new part which can detect cadmium ions in liquid.

BBa_K404300
BBa_K404300 Version 1 (Component)
SEG-Linker

BBa_K1607011
BBa_K1607011 Version 1 (Component)
The SCFV of anti-p185her2/neu antibody chA21 linked with EGFP by a (Gly4Ser)3 linker

BBa_K1613017
BBa_K1613017 Version 1 (Component)
K1613017 is a new part which can detect lead (Pb) ions in the liquid.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.