BBa_I737020
BBa_I737020 Version 1 (Component)
PoPS->Agarase (Digests agar)

BBa_K1906004
BBa_K1906004 Version 1 (Component)
Elongation Factor Tu

BBa_I732014
BBa_I732014 Version 1 (Component)
[aTc] -> PoPS

BBa_K206107
BBa_K206107 Version 1 (Component)
PoPS -> GFP

BBa_J29049
BBa_J29049 Version 1 (Component)
PoPS -> tumble

BBa_K176082
BBa_K176082 Version 1 (Component)
cell density->PoPS: pCon 1.00->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPS

BBa_K176081
BBa_K176081 Version 1 (Component)
cell density->PoPS: pCon max->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPS

BBa_J29005
BBa_J29005 Version 1 (Component)
PoPs->CrtI protein generator

BBa_J29004
BBa_J29004 Version 1 (Component)
PoPS ->CrtB protein generator

BBa_K1906000
BBa_K1906000 Version 1 (Component)
Elongation Factor Ts

BBa_K176162
BBa_K176162 Version 1 (Component)
PoPS->RBS(weak)+lacZalpha-ccdB-LVA->PoPS

BBa_K1675016
BBa_K1675016 Version 1 (Component)
P-atp2 mutant 140-B0034-lacz alpha

BBa_M36363
BBa_M36363 Version 1 (Component)
PoPS -> DbjA

BBa_K1920002
BBa_K1920002 Version 1 (Component)
PoPS->lysis

BBa_K1920004
BBa_K1920004 Version 1 (Component)
PoPS->lysis

BBa_K1920005
BBa_K1920005 Version 1 (Component)
PoPS->lysis

BBa_K1920003
BBa_K1920003 Version 1 (Component)
PoPS->lysis

BBa_J58115
BBa_J58115 Version 1 (Component)
PoPS -> CRP

BBa_K1072004
BBa_K1072004 Version 1 (Component)
alpha-ALS Coding Region

BBa_K2185002
BBa_K2185002 Version 1 (Component)
Penicillium alpha amylase coding sequence

BBa_J37027
BBa_J37027 Version 1 (Component)
Pops Blocker (Cre/Lox system)

BBa_K094170
BBa_K094170 Version 1 (Component)
lacZ alpha

BBa_J17001
BBa_J17001 Version 1 (Component)
G-alpha-olf

BBa_K1891010
BBa_K1891010 Version 1 (Component)
YCE+alpha-tubulin

BBa_K782060
BBa_K782060 Version 1 (Component)
Interferon alpha-2a

402
BBa_K398402 Version 1 (Component)
Prefoldin-alpha generator

BBa_K1891009
BBa_K1891009 Version 1 (Component)
YNE+alpha-tubulin

BBa_J17003
BBa_J17003 Version 1 (Component)
G-alpha-olf

BBa_K1891007
BBa_K1891007 Version 1 (Component)
alpha-tubulin+YNE

BBa_K1891008
BBa_K1891008 Version 1 (Component)
alpha-tubulin+YCE

BBa_K539742
BBa_K539742 Version 1 (Component)
TetR repressible promoter (BBa_R0040)+ kivd (alpha-ketoisovalerate decarboxylase)+Ter (BBa_B0015)

BBa_M36017
BBa_M36017 Version 1 (Component)
PoPS -> Puprle

BBa_J64100
BBa_J64100 Version 1 (Component)
Medium copy ColE1 BioBrick vector

BBa_K176192
BBa_K176192 Version 1 (Component)
PoPS->GFP->CI Inverter(cI-LVA+pCI)->luxI-LVA->PoPS

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K2185006
BBa_K2185006 Version 1 (Component)
S.ferax alpha amylase coding sequence

BBa_K176148
BBa_K176148 Version 1 (Component)
cell density->PoPS: pCon 0.15->luxI-LVA+pCon 0.36->luxR+pLux/Tet->PoPS

BBa_K176154
BBa_K176154 Version 1 (Component)
cell density->PoPS: pCon 0.01->luxI-LVA+pCon 0.36->luxR+pLux/Tet->PoPS

BBa_K176151
BBa_K176151 Version 1 (Component)
cell density->PoPS: pCon 0.04->luxI-LVA+pCon 0.36->luxR+pLux/Tet->PoPS

BBa_K176145
BBa_K176145 Version 1 (Component)
cell density->PoPS: pCon 0.36->luxI-LVA+pCon 0.36->luxR+pLux/Tet->PoPS

BBa_K176194
BBa_K176194 Version 1 (Component)
cell density->PoPS: pCon 0.70->luxI-LVA+pCon 0.36->luxR+pLux/Tet->PoPS

Spo0A
BBa_J01004 Version 1 (Component)
Spo0A Transcription Factor

BBa_K2143001
BBa_K2143001 Version 1 (Component)
NahR_L154F Transcription Factor

BBa_M36808
BBa_M36808 Version 1 (Component)
PoPS -> Cell Lysis

BBa_K362004
BBa_K362004 Version 1 (Component)
sodA promoter is controlled by some transcription factors including SoxS.

BBa_P0351
BBa_P0351 Version 1 (Component)
PoPS -> cI (lambda) [S0159]

BBa_J13003
BBa_J13003 Version 1 (Component)
POPS+RIPS generates CFP. POPS generates YFP

BBa_P2007
BBa_P2007 Version 1 (Component)
PoPS->Zif268-GCN4.MalE

BBa_K1871001
BBa_K1871001 Version 1 (Component)
Alpha amylase with native secretion

BBa_R4032
BBa_R4032 Version 1 (Component)
PoPS/RiPS Generator. Subparts: R0040. B0032