BBa_S01936
BBa_S01936 Version 1 (Component)
Intermediate part from assembly 240

BBa_S02107
BBa_S02107 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01996
BBa_S01996 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01376
BBa_S01376 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01379
BBa_S01379 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01663
BBa_S01663 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01660
BBa_S01660 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01898
BBa_S01898 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01381
BBa_S01381 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01830
BBa_S01830 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01845
BBa_S01845 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01380
BBa_S01380 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01377
BBa_S01377 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01842
BBa_S01842 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01383
BBa_S01383 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01843
BBa_S01843 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01378
BBa_S01378 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01826
BBa_S01826 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01382
BBa_S01382 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01821
BBa_S01821 Version 1 (Component)
Intermediate part from assembly 240

BBa_K323164
BBa_K323164 Version 1 (Component)
VioA and VioB enzymes fused with zinc fingers under pBAD promoter in pSB4K5

BBa_S02001
BBa_S02001 Version 1 (Component)
Intermediate part from assembly 240

BBa_K619894
BBa_K619894 Version 1 (Component)
mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K1447004
BBa_K1447004 Version 1 (Component)
Epitope 1-5 from Ara h 1

BBa_K1447005
BBa_K1447005 Version 1 (Component)
Epitope 1-5 from Ara h 1

BBa_K619895
BBa_K619895 Version 1 (Component)
mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K833002
BBa_K833002 Version 1 (Component)
mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K833006
BBa_K833006 Version 1 (Component)
Mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K833008
BBa_K833008 Version 1 (Component)
Mutated prfA-UTR thermosensor from Listeria monocytogenes

BBa_K323163
BBa_K323163 Version 1 (Component)
VioC, VioD and VioE enzymes fused with zinc fingers under pBAD promoter in pSB4C5

PPK
BBa_K986000 Version 1 (Component)
This is a PPK Coding Sequence which can remove phosphate from water

BBa_K2066500
BBa_K2066500 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

BBa_K1323020
BBa_K1323020 Version 1 (Component)
oriV from the Staphylococcus aureus pSK41 plasmid clone 3

BBa_K1497197
BBa_K1497197 Version 1 (Component)
B0034-CHI - Chalcone Isomerase from Petunia with strong RBS

BBa_K1465203
BBa_K1465203 Version 1 (Component)
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from Synechococcus elongatus

BBa_K1968014
BBa_K1968014 Version 1 (Component)
Tcyc Phytobrick: cytochrome C gene transcriptional terminator from Saccharomyces cerevisiae

BBa_K294205
BBa_K294205 Version 1 (Component)
This is a coding sequence of heat shock protein from E.coli

BBa_K2092004
BBa_K2092004 Version 1 (Component)
alcR (incl RBS), ethanol-activated transcription factor from A. nidulans

BBa_K1968009
BBa_K1968009 Version 1 (Component)
PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger

BBa_K1968013
BBa_K1968013 Version 1 (Component)
PgdaA constituve Phytobrick promoter: Glyceraldehyde-3-phosphate dehydrogenase from Aspergillus nige

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.