BBa_K758003
BBa_K758003 Version 1 (Component)
UAS, this part consists of five UAS sequences and hsp70 TATA.

BBa_K758004
BBa_K758004 Version 1 (Component)
GAL4, this part coding protein binds and activates the UAS sequence

BBa_S01304
BBa_S01304 Version 1 (Component)
Intermediate part from assembly 236

UAS-LacZ
BBa_K758005 Version 1 (Component)
UAS LacZ, this part expresses LacZ in the presence of GAL4

UAS-EGFP
BBa_K758006 Version 1 (Component)
UAS EGFP, this part expresses EGFP in the presence of GAL4 protein.

BBa_S03419
BBa_S03419 Version 1 (Component)
--Specify Parts List--

BBa_K737003
BBa_K737003 Version 1 (Component)
This part is polyphosphate kinase.

BBa_K737016
BBa_K737016 Version 1 (Component)
This part conteins gvpA protein's coding sequence.

BBa_K737017
BBa_K737017 Version 1 (Component)
This part conteins gvpC-20psi protein

BBa_K737022
BBa_K737022 Version 1 (Component)
This part is gvpC-20 coding gene with GFP mut 3.1 fusion gene

BBa_K739004
BBa_K739004 Version 1 (Component)
Ax21 protein - signaling part

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

BBa_K774002
BBa_K774002 Version 1 (Component)
Comparator Circuit Part 1

BBa_K774003
BBa_K774003 Version 1 (Component)
Comparator Circuit Part 2

BBa_K113021
BBa_K113021 Version 1 (Component)
Total construct: T7 polymerase + GFP/RFP + repressors + overlapping T7 promoter

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

Cello E. Coli Parts
Eco1C1G1T1_collection Version 1 (Collection)
These are the Cello parts for E. Coli circuits

BBa_K143077
BBa_K143077 Version 1 (Component)
P43-gsiB GFP expression construct

BBa_K143078
BBa_K143078 Version 1 (Component)
P43-spoVG GFP expression construct

BBa_K143079
BBa_K143079 Version 1 (Component)
Pveg-spoVG GFP expression construct

BBa_K143080
BBa_K143080 Version 1 (Component)
P43-gsiB RFP expression construct

BBa_K143081
BBa_K143081 Version 1 (Component)
P43-spoVG RFP expression construct

BBa_K143082
BBa_K143082 Version 1 (Component)
Pveg-spoVG RFP expression construct

BBa_K082000
BBa_K082000 Version 1 (Component)
reconstruct strong RBS

Regional
BBa_K106676 Version 1 (Component)
Regional silencing construct

BBa_S04142
BBa_S04142 Version 1 (Component)
The Partial Butanol Operon (Parts I725021+I725022)

BBa_S04143
BBa_S04143 Version 1 (Component)
The Partial Butanol Operon (Parts I725023+I725024)

BBa_S04144
BBa_S04144 Version 1 (Component)
The Partial Butanol Operon (Parts I725024+I725025)

BBa_S04145
BBa_S04145 Version 1 (Component)
The Partial Butanol Operon (Parts I725021+I725022+I725023)

BBa_K106671
BBa_K106671 Version 1 (Component)
GFP, Aar1 AD part

BBa_K106677
BBa_K106677 Version 1 (Component)
Ssn8, Aar1 AD part

BBa_S04147
BBa_S04147 Version 1 (Component)
The Partial Butanol Operon (5 inserts from I725021 to I725025)

BBa_K106692
BBa_K106692 Version 1 (Component)
Sir3, AarI A!D part (A!D variant!)

BBa_K112410
BBa_K112410 Version 1 (Component)
cadC promoter and GFP reporter construct

BBa_K152010
BBa_K152010 Version 1 (Component)
TB1 RNAi construct

BBa_K152011
BBa_K152011 Version 1 (Component)
GFP RNAi construct

BBa_K112409
BBa_K112409 Version 1 (Component)
RecA reporter GFP construct

BBa_K112411
BBa_K112411 Version 1 (Component)
cadAB promoter GFP reporter construct

BBa_K112412
BBa_K112412 Version 1 (Component)
grpE promoter GFP reporter construct

BBa_K112413
BBa_K112413 Version 1 (Component)
ptet promoter GFP reporter construct

BBa_K112414
BBa_K112414 Version 1 (Component)
spv promoter and GFP reporter construct

BBa_K116646
BBa_K116646 Version 1 (Component)
Intermediate part to construct a synchronizer

BBa_K116648
BBa_K116648 Version 1 (Component)
Intermediate part to construct a synchronizer

BBa_K093014
BBa_K093014 Version 1 (Component)
Nuclease construct

BBa_J70030
BBa_J70030 Version 1 (Component)
A BioScaffold Part (Uses PpiI), Protein Tail Remover see Part Design Page

BBa_J70031
BBa_J70031 Version 1 (Component)
Linker for BioScaffold Parts, example of Gamma BioScaffold part

BBa_J70033
BBa_J70033 Version 1 (Component)
A BioMortar Part for Creating GSGS Protein Fusions (see Design page)

BBa_J70034
BBa_J70034 Version 1 (Component)
A BioScaffold Part (Uses AarI) for Library Vector Preparation (see Part Design page)