BBa_K535005BBa_K535005 Version 1 (Component)HydA1 - FeOx construction (Hydrogenase-ferredoxin fussion)
BBa_K615004BBa_K615004 Version 1 (Component)Zif268 binding site-His3-URA3 construct for one-hybrid selection
BBa_S01291BBa_S01291 Version 1 (Component)Intermediate part from assembly 236
BBa_S01293BBa_S01293 Version 1 (Component)Intermediate part from assembly 236
BBa_S01294BBa_S01294 Version 1 (Component)Intermediate part from assembly 236
BBa_S01295BBa_S01295 Version 1 (Component)Intermediate part from assembly 236
BBa_S01296BBa_S01296 Version 1 (Component)Intermediate part from assembly 236
BBa_S01297BBa_S01297 Version 1 (Component)Intermediate part from assembly 236
BBa_S01298BBa_S01298 Version 1 (Component)Intermediate part from assembly 236
BBa_S01299BBa_S01299 Version 1 (Component)Intermediate part from assembly 236
BBa_S01300BBa_S01300 Version 1 (Component)Intermediate part from assembly 236
BBa_S01301BBa_S01301 Version 1 (Component)Intermediate part from assembly 236
BBa_S01302BBa_S01302 Version 1 (Component)Intermediate part from assembly 236
BBa_J72209BBa_J72209 Version 1 (Component)kanamycin + ampicillin resistant p15A vector for 2ab assembly
BBa_S01303BBa_S01303 Version 1 (Component)Intermediate part from assembly 236
BBa_S01304BBa_S01304 Version 1 (Component)Intermediate part from assembly 236
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
BBa_K113021BBa_K113021 Version 1 (Component)Total construct: T7 polymerase + GFP/RFP + repressors + overlapping T7 promoter
BBa_K143071BBa_K143071 Version 1 (Component)AmyE integratable PoPS generator (P43-gsiB) (with CmR)
BBa_K143072BBa_K143072 Version 1 (Component)AmyE integratable PoPS generator (P43-spoVG) (with CmR)
BBa_K143073BBa_K143073 Version 1 (Component)AmyE integratable PoPS generator (Pveg-gsiB) (with CmR)
BBa_K143074BBa_K143074 Version 1 (Component)AmyE integratable PoPS generator (Pveg-spoVG) (with CmR)
BBa_K143077BBa_K143077 Version 1 (Component)P43-gsiB GFP expression construct
BBa_K143078BBa_K143078 Version 1 (Component)P43-spoVG GFP expression construct
BBa_K143079BBa_K143079 Version 1 (Component)Pveg-spoVG GFP expression construct
BBa_K143080BBa_K143080 Version 1 (Component)P43-gsiB RFP expression construct
BBa_K143081BBa_K143081 Version 1 (Component)P43-spoVG RFP expression construct
BBa_K143082BBa_K143082 Version 1 (Component)Pveg-spoVG RFP expression construct
BBa_K082000BBa_K082000 Version 1 (Component)reconstruct strong RBS
RegionalBBa_K106676 Version 1 (Component)Regional silencing construct
BBa_I13601BBa_I13601 Version 1 (Component)Lac operator with CFP reporter (without LVA tag) [R/Lc-]
BBa_K112410BBa_K112410 Version 1 (Component)cadC promoter and GFP reporter construct
BBa_K116646BBa_K116646 Version 1 (Component)Intermediate part to construct a synchronizer
BBa_K116648BBa_K116648 Version 1 (Component)Intermediate part to construct a synchronizer
BBa_K152010BBa_K152010 Version 1 (Component)TB1 RNAi construct
BBa_K152011BBa_K152011 Version 1 (Component)GFP RNAi construct
BBa_K112409BBa_K112409 Version 1 (Component)RecA reporter GFP construct
BBa_K112411BBa_K112411 Version 1 (Component)cadAB promoter GFP reporter construct
BBa_K112412BBa_K112412 Version 1 (Component)grpE promoter GFP reporter construct
BBa_K112413BBa_K112413 Version 1 (Component)ptet promoter GFP reporter construct
BBa_K112414BBa_K112414 Version 1 (Component)spv promoter and GFP reporter construct
BBa_K112134BBa_K112134 Version 1 (Component)Cam-Amp assembly vector
BBa_K093014BBa_K093014 Version 1 (Component)Nuclease construct
BBa_J70102BBa_J70102 Version 1 (Component)Left 6 his test part for library construction part.
BBa_J70104BBa_J70104 Version 1 (Component)Right 6 his test part for library construction part.
pSB1C4pSB1C4 Version 1 (Component)High copy BioBrick assembly plasmid
BBa_J69604BBa_J69604 Version 1 (Component)lacI Inverter Test Construct A
BBa_J69616BBa_J69616 Version 1 (Component)tetR Inverter Test Construct B
BBa_P111101BBa_P111101 Version 1 (Component)SAMT Whole construct