RFC105 NC
BBa_K1362417 Version 1 (Component)
C-terminal GG-linker overhang GG<u>TGGT</u>=Gly+Gly RFC[105] overhang NC

RFC105 Z
BBa_K1362421 Version 1 (Component)
C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z

<- BsaI
BBa_K1362423 Version 1 (Component)
BsaI reverse restriction site for RFC[105] cloning

BsaI ->
BBa_K1362424 Version 1 (Component)
BsaI restriction site for RFC[105] cloning

1Gate-NpuD
BBa_K1362100 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] assembly construct (with His6)

RFC105 A
BBa_K1362414 Version 1 (Component)
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A

BBa_S01649
BBa_S01649 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01653
BBa_S01653 Version 1 (Component)
Intermediate part from assembly 240

BBa_K1357003
BBa_K1357003 Version 1 (Component)
IPTG Inducible NhaR Expression Construct

NpuDnaE(C)
BBa_K1362401 Version 1 (Component)
NpuDnaE C-Intein cloning piece

SspDnaX(C)
BBa_K1362403 Version 1 (Component)
SspDnaX C-Intein cloning piece

TerDnaE3(C
BBa_K1362405 Version 1 (Component)
TerDnaE3 C-Intein cloning piece

SspGyrB(C)
BBa_K1362407 Version 1 (Component)
SspGyrB C-Intein cloning piece

SspDnaE(C)
BBa_K1362411 Version 1 (Component)
SspDnaE C-Intein cloning piece

SspDnaB(C)
BBa_K1362409 Version 1 (Component)
SspDnaB C-Intein cloning piece

Gp41-1(C)
BBa_K1362413 Version 1 (Component)
Gp41-1 C-Intein cloning piece

BBa_S01660
BBa_S01660 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01663
BBa_S01663 Version 1 (Component)
Intermediate part from assembly 240

RFC105 NN*
BBa_K1362416 Version 1 (Component)
N-terminal non-splicing overhang GGCT=Gly+1/3Leu|Ile RFC[105] overhang NN*

RFC105 CN
BBa_K1362418 Version 1 (Component)
N-terminal GG-linker overhang <u>GGTG</u>=Gly+1/3Gly RFC[105] overhang CN

RFC105 CC
BBa_K1362419 Version 1 (Component)
C-terminal splicing overhang CAAC=1/3?+Asn RFC[105] standard overhang CC

RFC[105] E
BBa_K1362420 Version 1 (Component)
C-terminal non-splicing overhang AGGC=1/3Ala+Gly RFC[105] E

CircNpu
BBa_K1362000 Version 1 (Component)
NpuDnaE intein RFC[105] circularization construct

CircNpu*
BBa_K1362001 Version 1 (Component)
NpuDnaE intein RFC[105] circularization construct (with FLAG and Smt3)

His6
BBa_K1362431 Version 1 (Component)
N-terminal His6 (C-Intein)

BBa_K1362204
BBa_K1362204 Version 1 (Component)
Sortase A non-circularization construct (with His6; without RBS)

BBa_K1362205
BBa_K1362205 Version 1 (Component)
Sortase A non-circularization construct (with Smt3 and His6; without RBS)

BBa_K1362203
BBa_K1362203 Version 1 (Component)
Sortase A circularization construct (with Smt3 and His6; without RBS)

BBa_K1362202
BBa_K1362202 Version 1 (Component)
Sortase A circularization construct (with His6; without RBS)

BBa_K1362052
BBa_K1362052 Version 1 (Component)
Carrier for RFC[105] C-terminal inserts

N-carrier
BBa_K1362053 Version 1 (Component)
Carrier for RFC[105] N-terminal inserts

BBa_K1362054
BBa_K1362054 Version 1 (Component)
SsrA degradation tag as RFC[105] C-terminal insert

BBa_K1362901
BBa_K1362901 Version 1 (Component)
C-terminal tripleSWAGtag (C-intein)

RFC105 A
BBa_K1362440 Version 1 (Component)
N-terminal start overhang (T)(A)-GATG=RBS+Start RFC[105] A

Smt3-mDNMT
BBa_K1362500 Version 1 (Component)
Smt3-Murine DNMT1(731-1602) fusion protein (with His6)

BBa_K1384000
BBa_K1384000 Version 1 (Component)
silk masp2 monomer for ICA assembly

BBa_K1362055
BBa_K1362055 Version 1 (Component)
MinD MTS as RFC[105] C-terminal insert

BBa_K1362056
BBa_K1362056 Version 1 (Component)
Myristoilation signal sequence as RFC[105] N-terminal insert

BBa_K1362057
BBa_K1362057 Version 1 (Component)
NLS as RFC[105] N-terminal insert

BBa_K1362058
BBa_K1362058 Version 1 (Component)
PelB leading sequence as RFC[105] N-terminal insert

BBa_K1362059
BBa_K1362059 Version 1 (Component)
PKI-NES as RFC[105] N-terminal insert

BBa_K1362060
BBa_K1362060 Version 1 (Component)
OmpA surface protein as RFC[105] N-terminal insert

BBa_K1362399
BBa_K1362399 Version 1 (Component)
mutated (c-terminal Ser ->Ala) NpuDnaE C-Intein cloning piece

BBa_K1362023
BBa_K1362023 Version 1 (Component)
RBS + Xylanase (with His6)

BBa_K1362106
BBa_K1362106 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] assembly construct with additional C-teminal insertion site

1NpuE(N*)
BBa_K1362102 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] non-splicing control construct (with His6)

1GateNpuE
BBa_K1362101 Version 1 (Component)
RBS+NpuDnaE C-intein RFC[105] assembly construct (with His6)

BBa_K1362107
BBa_K1362107 Version 1 (Component)
RBS + NpuDnaE C-intein RFC[105] assembly construct with additional N-teminal insertion site

1NpuE(C*)
BBa_K1362103 Version 1 (Component)
RBS + NpuDnaE C-intein RFC[105] non-slicing control construct (with His6)

TerDnaE3(N
BBa_K1362130 Version 1 (Component)
RBS + TerDnaE3 N-intein RFC[105] assembly construct (with His6)