BBa_K1916003
BBa_K1916003 Version 1 (Component)
Cha6605_4344g2 + intein-CBD tag

BBa_K1916006
BBa_K1916006 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1435000
BBa_K1435000 Version 1 (Component)
4-HT inducible intein

BBa_K1916002
BBa_K1916002 Version 1 (Component)
Cyan7427_1390g3 + intein-CBD tag

BBa_K1916021
BBa_K1916021 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916004
BBa_K1916004 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916016
BBa_K1916016 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916012
BBa_K1916012 Version 1 (Component)
CBCR3 + intein-CBD tag

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J07048
BBa_J07048 Version 1 (Component)
FecA::scFv internal fusion type 1 @ aa155

BBa_J07053
BBa_J07053 Version 1 (Component)
FecA::scFv internal fusion type 1 @ aa571

BBa_J07049
BBa_J07049 Version 1 (Component)
FecA::scFv internal fusion type 1 @ aa176

BBa_J07052
BBa_J07052 Version 1 (Component)
FecA::scFv internal fusion type 1 @ aa527

BBa_K1314016
BBa_K1314016 Version 1 (Component)
SH3 Domain-Linker-PDZ Domain

BBa_K077555
BBa_K077555 Version 1 (Component)
part 1 for interval switch

BBa_K1362160
BBa_K1362160 Version 1 (Component)
RBS + Gp41-1 N-intein RFC[105] assembly construct (with His6)

RGK
BBa_K1362434 Version 1 (Component)
RGK (extein)

BBa_K1679022
BBa_K1679022 Version 1 (Component)
KIG, kcoil+intein+gfp

BBa_K341000
BBa_K341000 Version 1 (Component)
recombination sequence + eGFP+I-Sce I cutting site

BBa_K1976048
BBa_K1976048 Version 1 (Component)
DNase domain of Colicin E2

3xSWAG(N)
BBa_K1362900 Version 1 (Component)
N-terminal tripleSWAGtag (N-intein)

BBa_K1362901
BBa_K1362901 Version 1 (Component)
C-terminal tripleSWAGtag (C-intein)

BBa_J70012
BBa_J70012 Version 1 (Component)
A BioScaffold Part (Uses PsrI), Protein Head Remover see Part Design page

BBa_J56006
BBa_J56006 Version 1 (Component)
Codon-optimized signaling domain, cytoplasmic domain of Tar

BBa_K371056
BBa_K371056 Version 1 (Component)
ATG

GFP-AID
BBa_K812010 Version 1 (Component)
GFP fused with AID-tag (ubuquitinase E3 OsTirI recoginition domain)

BBa_K1909004
BBa_K1909004 Version 1 (Component)
NOD1 LRR Domain

BBa_K1491027
BBa_K1491027 Version 1 (Component)
T7 RNA Polymerase with Dead ER Intein

BBa_K341005
BBa_K341005 Version 1 (Component)
recombination sequence + Kan+I-Sce I cutting site

BBa_K341006
BBa_K341006 Version 1 (Component)
recombination sequence +chlr+I-Sce I cutting site

BBa_K341004
BBa_K341004 Version 1 (Component)
recombination sequence +mCherry+I-Sce I cutting site

SspDnaB(N*
BBa_K1362142 Version 1 (Component)
RBS + SspDnaB N-intein RFC[105] non-splicing control construct (with His6)

BBa_K845006
BBa_K845006 Version 1 (Component)
Tap extracellular domain

BBa_J07060
BBa_J07060 Version 1 (Component)
FecA::scFv internal fusion type 2 @ aa522-532

BBa_J07063
BBa_J07063 Version 1 (Component)
FecA::scFv internal fusion type 2 @ aa566-577

BBa_K1890053
BBa_K1890053 Version 1 (Component)
INP transmembrane domain

BBa_I714921
BBa_I714921 Version 1 (Component)
SDE'_EGFP

BBa_K1641999
BBa_K1641999 Version 1 (Component)
pAUR135 shuttle vector RFC10 optimized

BBa_K608405
BBa_K608405 Version 1 (Component)
plastic binding domain

BBa_K2022002
BBa_K2022002 Version 1 (Component)
TolR, Periplasmic Domain

BBa_K1351007
BBa_K1351007 Version 1 (Component)
CWB domain of B. subtilis minor autolysin LytE

BBa_K1051704
BBa_K1051704 Version 1 (Component)
Mer2 Intron

BBa_K1791006
BBa_K1791006 Version 1 (Component)
MS2 binding domain

BBa_K826004
BBa_K826004 Version 1 (Component)
oprf coding domain

BBa_M1150
BBa_M1150 Version 1 (Component)
SBE Class

BBa_K077666
BBa_K077666 Version 1 (Component)
part 2 for interval switch

BBa_K2017000
BBa_K2017000 Version 1 (Component)
C-split Cas9 + DnaE C-intein

BBa_J07051
BBa_J07051 Version 1 (Component)
FecA::scFv internal fusion type 1 @ aa180

BBa_J07047
BBa_J07047 Version 1 (Component)
FecA::scFv internal fusion type 1 @ aa138